PMID: 3768210Jan 1, 1986Paper

An ultrastructural study of osteogenesis in chick periosteum in vitro

Bone
H C TenenbaumK P Pritzker

Abstract

We described a chick periosteal osteogenesis model at the light microscopic level and biochemically. To confirm that morphologically identifiable bone is being formed, we performed an ultrastructural study of this model. Samples of freshly dissected periostea, as well as whole calvariae, were fixed in glutaraldehyde. Cultured tissues were subsequently harvested and similarly fixed at 2, 4, and 6 days of culture. Examination of freshly dissected periostea showed that these tissues contained many fibroblast-like cells within a loosely arranged connective tissue. There was no evidence of osteoid or mineral in this tissue. Whole calvarial explants contained well-mineralized bone surrounded by an osteoid seam. At 2 days in culture there was an increase in cellular organization as well as extracellular matrix production. No mineral was detected. By 4 days a non-mineralized osteoid matrix was present, which consisted of densely packed, highly oriented, crossbanded collagen fibrils. Early spherical mineral deposits were seen. The osteoid was surrounded by a multilayer of osteoblast-like cells. The 6-day cultures all contained well-mineralized matrix. The mineral was identified by electron diffraction to be apatite. The data suggest tha...Continue Reading

Citations

Sep 1, 1989·Cell and Tissue Research·H C TenenbaumC Birek
Jan 1, 1990·Calcified Tissue International·M Casser-BetteJ Schmidt
Apr 1, 1997·In Vitro Cellular & Developmental Biology. Animal·D Lovitch, M L Christianson
Jan 1, 1988·Bone·A van der Plas, P J Nijweide
Sep 27, 2003·Tissue Engineering·Dietmar W Hutmacher, Michael Sittinger
Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D Lovitch, M L Christianson
Sep 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M ThomasJ D Best
Dec 9, 2008·Journal of Biomechanics·Jasper FoolenKeita Ito
Jun 23, 2009·Biochemical and Biophysical Research Communications·Aya KawanamiShunichi Murakami
Apr 13, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Seungyoun LyuXinping Zhang
Jan 1, 1989·Connective Tissue Research·M D GrynpasD P Holmyard
May 2, 2015·The Journal of Craniofacial Surgery·Ki-Deog ParkHongju Park
Jun 27, 2003·The Journal of Craniofacial Surgery·Wanda E OpreaJohn E Davies

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