PMID: 12756326May 21, 2003Paper

An uncapped RNA suggests a model for Caenorhabditis elegans polycistronic pre-mRNA processing

RNA
Yingmiao LiuThomas Blumenthal

Abstract

Polycistronic pre-mRNAs from Caenohabditis elegans operons are processed by internal cleavage and polyadenylation to create 3' ends of mature mRNAs. This is accompanied by trans-splicing with SL2 approximately 100 nucleotides downstream of the 3' end formation sites to create the 5' ends of downstream mRNAs. SL2 trans-splicing depends on a U-rich element (Ur), located approximately 70 nucleotides upstream of the trans-splice site in the intercistronic region (ICR), as well as a functional 3' end formation signal. Here we report the existence of a novel gene-length RNA, the Ur-RNA, starting just upstream of the Ur element. The expression of Ur-RNA is dependent on 3' end formation as well as on the presence of the Ur element, but does not require a trans-splice site. The Ur-RNA is not capped, and alteration of the location of the Ur element in either the 5' or 3' direction alters the location of the 5' end of the Ur-RNA. We propose that a 5' to 3' exonuclease degrades the precursor RNA following cleavage at the poly(A) site, stopping when it reaches the Ur element, presumably attributable to a bound protein. Part of the function of this protein can be performed by the MS2 coat protein. Recruitment of coat protein to the ICR in th...Continue Reading

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Citations

Mar 31, 2005·Trends in Genetics : TIG·Kenneth E M Hastings
Sep 11, 2009·Nucleic Acids Research·Simon HaenniAndré Furger
Jul 14, 2010·Genes & Development·Erika L LasdaThomas Blumenthal
Dec 29, 2004·Cell·Weifei Luo, David Bentley
Apr 29, 2016·Transcription·Alfonso Garrido-LeccaThomas Blumenthal
Nov 7, 2015·Journal of Cell Science·Andrew J FridayBrett D Keiper

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