PMID: 7017387Apr 1, 1981Paper

An unexpected response to Torulopsis glabrata fusion products to X-irradiation

Mutation Research
C L GaleottiG D Clark-Walker

Abstract

Intra-species fusion products of Saccharomyces cerevisiae, Saccharomyces unisporus and Torulopsis glabrata have been isolated following polyethylene glycol-induced fusion of protoplasts and selection for prototrophic colonies. Staining with lomofungin showed that all fusion products were uninucleate. Measurement of DNA content mostly gave values between haploid and diploid levels indicating that the majority of fusion products were aneuploid, Nevertheless fusion products of S. cerevisiae and S. unisporus were, as expected, more resistant to X-irradiation than their haploid parents. By contrast, the X-ray dose-response curve of all T. glabrata fusion products was indistinguishable from their progenitors despite the fact that mitotic segregants could be recovered amongst the survivors to X-rays. A possible explanation for the behaviour towards X-rays of T. glabrata fusion products is that this species lacks a DNA repair pathway involving recombination between homologous chromosomes. We conclude from this study that the shape of the X-ray dose-response curve should not be taken to indicate the ploidy of new yeast isolates without supporting data.

References

Jul 4, 1978·Molecular & General Genetics : MGG·G Brunborg, D H Williamson
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Jun 1, 1976·Journal of Theoretical Biology·M A Resnick
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Citations

Feb 1, 1984·Current Genetics·K S Sriprakash, C Batum
Jan 1, 1987·Critical Reviews in Microbiology·W L Whelan
Jan 1, 1987·Critical Reviews in Microbiology·R O NicholasF J Wayman
Oct 1, 1989·Journal of Clinical Microbiology·C S Kaufmann, W G Merz
Nov 1, 1987·Journal of Bacteriology·W L Whelan, K J Kwon-Chung

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