An unstable competence-induced protein, CoiA, promotes processing of donor DNA after uptake during genetic transformation in Streptococcus pneumoniae

Journal of Bacteriology
Bhushan V Desai, Donald A Morrison

Abstract

Natural genetic transformation in Streptococcus pneumoniae entails transcriptional activation of at least two sets of genes. One set of genes, activated by the competence-specific response regulator ComE, is involved in initiating competence, whereas a second set is activated by the competence-specific alternative sigma factor ComX and functions in DNA uptake and recombination. Here we report an initial characterization of CoiA, a ComX-dependent gene product that is induced during competence and is required for transformation. CoiA is widely conserved among gram-positive bacteria, and in streptococci, the entire coiA locus composed of four genes is conserved. By use of immunoblot assay, we show that, similar to its message, CoiA protein is transient, appearing at 10 min and largely disappearing by 30 min post-competence induction. Using complementation analysis, we establish that coiA is the only gene of this induced locus needed for transformability. We find no indication of CoiA having a role in regulating competence. Finally, using 32P- and 3H-labeled donor DNA, we demonstrate that a coiA mutant can internalize normal amounts of donor DNA compared to the wild-type strain but is unable to process it into viable transformants,...Continue Reading

References

Aug 1, 1988·Molecular & General Genetics : MGG·V Méjean, J P Claverys
Apr 11, 1973·Biochimica Et Biophysica Acta·D A Morrison, W R Guild
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S LacksM Neuberger
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·N B Shoemaker, W R Guild
Nov 1, 1983·Journal of Bacteriology·M N Vijayakumar, D A Morrison
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·Leiv Sigve HavarsteinD A Morrison
Feb 1, 1997·Molecular Microbiology·Q ChengH R Masure
Apr 16, 1998·Molecular Microbiology·E A CampbellH R Masure
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Oct 27, 2001·Applied and Environmental Microbiology·C K SungD A Morrison
Jan 26, 2002·Nucleic Acids Research·Shyam UnniramanValakunja Nagaraja
Feb 7, 2002·Journal of Microbiological Methods·Peter C Y LauDennis G Cvitkovitch
Jul 19, 2002·Molecular Microbiology·Mathieu BergéJean-Pierre Claverys
Jul 23, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Jean-Pierre Claverys, Leiv Sigve Havarstein
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·A TOMASZ, R D HOTCHKISS
Jan 1, 1928·The Journal of Hygiene·F Griffith

Citations

Feb 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicolas MirouzeJean-Pierre Claverys
May 29, 2012·Nucleic Acids Research·Kamil SteczkiewiczKrzysztof Ginalski
Jul 3, 2007·Journal of Bacteriology·Donald A MorrisonJean-Pierre Claverys
Jul 17, 2007·Molecular Microbiology·Naomi KramerDavid Dubnau
Feb 11, 2014·Trends in Microbiology·Calum JohnstonJean-Pierre Claverys
Feb 21, 2009·FEMS Microbiology Reviews·Jean-Pierre ClaverysPatrice Polard
Sep 17, 2013·PLoS Pathogens·Matthieu J BergéJean-Pierre Claverys
Oct 11, 2012·Critical Reviews in Biochemistry and Molecular Biology·Dawit KidaneJ C Alonso
Jan 10, 2017·Frontiers in Microbiology·Edoardo ZaccariaJerry Wells
Mar 20, 2019·Journal of Bacteriology·Jelle SlagerJan-Willem Veening

Related Concepts

Bacterial Proteins
DNA Helix Destabilizing Proteins
DNA, Bacterial
Homologous Sequences, Nucleic Acid
Streptococcus
Streptococcus pneumoniae
Transformation, Bacterial
Determination, Sequence Homology
Conserved Sequence
Oligonucleotide Primers

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