An unstable targeted allele of the mouse Mitf gene with a high somatic and germline reversion rate.

Genetics
Keren BismuthHeinz Arnheiter

Abstract

The mouse Mitf gene encodes a transcription factor that is regulated by serine phosphorylation and is critical for the development of melanin-containing pigment cells. To test the role of phosphorylation at a particular serine, S73 in exon 2 of Mitf, we used a standard targeting strategy in mouse embryonic stem cells to change the corresponding codon into one encoding an alanine. By chance, we generated an allele in which 85,222 bp of wild-type Mitf sequence are duplicated and inserted into an otherwise correctly targeted Mitf gene. Depending on the presence or absence of a neomycin resistance cassette, this genomic rearrangement leads to animals with a white coat with or without pigmented spots or a gray coat with obligatory white and black spots. Several independent, genetically stable germline revertants that lacked the duplicated wild-type sequence but retained the targeted codon were then derived. These animals were normally pigmented, indicating that the serine-to-alanine mutation is not deleterious to melanocyte development. The fact that mosaic coat reversions occur in all mice lacking the neo-cassette and that approximately 1% of these transmit a reverted allele to their offspring places this mutation among those with ...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P K SeperackN G Copeland
Nov 1, 1995·Molecular and Cellular Biology·P De SepulvedaJ J Panthier
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y GondoM H Brilliant
Jan 1, 1996·Cytogenetics and Cell Genetics·A S DutraJ M Puck
Mar 21, 2000·Genesis : the Journal of Genetics and Development·M D Tallquist, P Soriano
Nov 10, 2000·Science·M Lynch, J S Conery
Feb 15, 2003·Genetics·Eiríkur SteingrímssonNancy A Jenkins
Jul 16, 2003·Pigment Cell Research·Dorothy C Bennett, M Lynn Lamoreux
Dec 1, 2004·Annual Review of Genetics·Eiríkur SteingrímssonNancy A Jenkins
Dec 1, 2004·Annual Review of Genetics·John S Taylor, Jeroen Raes
Dec 22, 2006·PloS One·Jeffery P DemuthMatthew W Hahn
Mar 28, 2007·Annual Review of Genomics and Human Genetics·Bernard Conrad, Stylianos E Antonarakis
Oct 1, 1992·Molecular and Cellular Neurosciences·M TachibanaH Arnheiter

❮ Previous
Next ❯

Citations

Oct 2, 2012·Cell Reports·Agathe ValluetAlain Eychène
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vanessa BessonGiovanna Marazzi
Feb 5, 2009·Pigment Cell & Melanoma Research·Thomas J HornyakFaith M Strickland
Aug 22, 2009·Pigment Cell & Melanoma Research·Corine Bertolotto, Robert Ballotti
Apr 9, 2010·Pigment Cell & Melanoma Research·Bin WenLing Hou
Mar 31, 2015·Pigment Cell & Melanoma Research·Claudia Wellbrock, Imanol Arozarena
Aug 18, 2018·Pigment Cell & Melanoma Research·Marc-Alexander RauschendorfJudith Fischer
Jun 12, 2012·Genetika·T V MarenkovaE V Deĭneko
May 28, 2019·Genes & Development·Colin R Goding, Heinz Arnheiter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.