An unusual choanoflagellate protein released by Hedgehog autocatalytic processing.

Proceedings. Biological Sciences
Elizabeth A SnellP W Holland

Abstract

Hedgehog proteins are important cell-cell signalling proteins utilized during the development of multicellular animals. Members of the hedgehog gene family have not been detected outside the Metazoa, raising unanswered questions about their evolutionary origin. Here we report a highly unusual hedgehog-related gene from a choanoflagellate, a close unicellular relative of the animals. The deduced C-terminal domain, Hoglet-C, is homologous to the autocatalytic domain of Hedgehog proteins and is predicted to function in autocatalytic cleavage of the precursor peptide. In contrast, the N-terminal Hoglet-N peptide has no similarity to the signalling peptide of Hedgehog (Hh-N). Instead, Hoglet-N is deduced to be a secreted protein with an enormous threonine-rich domain of unprecedented size and purity (over 200 threonine residues) and two polysaccharide-binding domains. Structural modelling reveals that these domains have a novel combination of features found in cellulose-binding domains (CBD) of types IIa and IIb, and are expected to bind cellulose. We propose that the two CBD domains enable Hoglet-N to bind to plant matter, tethering an amorphous nucleophilic anchor, facilitating transient adhesion of the choanoflagellate cell. Sinc...Continue Reading

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Citations

Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Atsuko SatoPeter W H Holland
Feb 15, 2013·Proceedings. Biological Sciences·Alan O MarronGiselle Walker
Oct 15, 2009·BMC Evolutionary Biology·Eve GazaveEmmanuelle Renard
Dec 2, 2008·Genome Biology·Thomas R Bürglin
May 8, 2008·PloS One·Kamran Shalchian-TabriziThomas Cavalier-Smith
Oct 18, 2008·Current Opinion in Genetics & Development·Antonis Rokas
Oct 22, 2008·The International Journal of Biochemistry & Cell Biology·Bernd SchierwaterRob Desalle
Feb 6, 2007·Trends in Genetics : TIG·Iñaki Ruiz-TrilloMichael W Gray
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Dec 20, 2014·Molecular Biology and Evolution·Xavier Grau-BovéIñaki Ruiz-Trillo
Sep 21, 2013·Annual Review of Genetics·Daniel J Richter, Nicole King
Dec 21, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Leslie S Babonis, Mark Q Martindale
Apr 20, 2011·Nature Reviews. Genetics·Philip W InghamClaudia Seger
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Mar 19, 2014·Chemical Science·Neel H Shah, Tom W Muir
Jan 11, 2008·Molecular Biology and Evolution·Iñaki Ruiz-TrilloB Franz Lang

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