An unusual metal-bound 4-fluorothreonine transaldolase from Streptomyces sp. MA37 catalyses promiscuous transaldol reactions.

Applied Microbiology and Biotechnology
Linrui WuHai Deng

Abstract

β-Hydroxy-α-amino acids (βH-AAs) are key components of many bioactive molecules as well as exist as specialised metabolites. Among these βH-AAs, 4-fluorothreonine (4-FT) is the only naturally occurring fluorinated AA discovered thus far. Here we report overexpression and biochemical characterisation of 4-fluorothreonine transaldolase from Streptomyces sp. MA37 (FTaseMA), a homologue of FTase previously identified in the biosynthesis of 4-FT in S. cattleya. FTaseMA displays considerable substrate plasticity to generate 4-FT as well as other β-hydroxy-α-amino acids with various functionalities at C4 position, giving the prospect of new chemo-enzymatic applications. The enzyme has a hybrid of two catalytic domains, serine hydroxymethyltransferase (S) and aldolase (A). Site-directed mutagenesis allowed the identification of the key residues of FTases, suggesting that the active site of A domain has a historical reminiscent feature in metal-dependent aldolases. Elemental analysis demonstrated that FTaseMA is indeed a Zn2+-dependent enzyme, the first example of pyridoxal phosphate (PLP) enzyme family fused with a metal-binding domain carrying out a distinct catalytic role. Finally, FTaseMA showed divergent evolutionary origin with ot...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Mar 22, 2002·Nature·David O'HaganCormac D Murphy
Oct 27, 2004·Amino Acids·R Bhushan, H Brückner
Nov 27, 2004·Natural Product Reports·Hai DengChristoph Schaffrath
Jan 21, 2010·Journal of Natural Products·Alessandra S EustáquioBradley S Moore
Sep 8, 2011·Alcoholism, Clinical and Experimental Research·Xiangying GuanHelen Anni
Nov 1, 2012·Journal of the American Chemical Society·Sandra Barnard-BritsonSteven G Van Lanen
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wonchull KangJin Kuk Yang
Jan 23, 2014·Chembiochem : a European Journal of Chemical Biology·Hai DengDavid O'Hagan
May 24, 2014·Nature Protocols·Wenxiu MaTimothy L Bailey
Sep 26, 2014·Chemical Reviews·David O'Hagan, Hai Deng
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Jul 5, 2016·Chemistry : a European Journal·Qingzhi ZhangDavid O'Hagan
May 16, 2017·Nature Chemical Biology·Jason E SchafferTimothy A Wencewicz
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jonathan L McMurry, Michelle C Y Chang
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jul 3, 2018·Chembiochem : a European Journal of Chemical Biology·Phillip T LoweDavid O'Hagan
Aug 8, 2018·Nature Communications·Guillaume A W BeaudoinAndrew D Hanson
Sep 6, 2018·Natural Product Reports·Yi-Ling Du, Katherine S Ryan

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Citations

Feb 9, 2021·Synthetic and Systems Biotechnology·Qing FangHai Deng
Dec 19, 2020·ACS Chemical Biology·Prasanth KumarAndrew R Buller
Sep 1, 2021·Trends in Biotechnology·Seon-Hwa LeeDeok-Kun Oh
Oct 10, 2021·Applied Microbiology and Biotechnology·Xinkuan Cheng, Long Ma

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Methods Mentioned

BETA
PCR
size exclusion chromatography

Software Mentioned

Excel
ClustalW
MEGA7

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