An Unusual Phage Repressor Encoded by Mycobacteriophage BPs

PloS One
Valerie M VillanuevaGraham F Hatfull

Abstract

Temperate bacteriophages express transcription repressors that maintain lysogeny by down-regulating lytic promoters and confer superinfection immunity. Repressor regulation is critical to the outcome of infection-lysogenic or lytic growth-as well as prophage induction into lytic replication. Mycobacteriophage BPs and its relatives use an unusual integration-dependent immunity system in which the phage attachment site (attP) is located within the repressor gene (33) such that site-specific integration leads to synthesis of a prophage-encoded product (gp33103) that is 33 residues shorter at its C-terminus than the virally-encoded protein (gp33136). However, the shorter form of the repressor (gp33103) is stable and active in repression of the early lytic promoter PR, whereas the longer virally-encoded form (gp33136) is inactive due to targeted degradation via a C-terminal ssrA-like tag. We show here that both forms of the repressor bind similarly to the 33-34 intergenic regulatory region, and that BPs gp33103 is a tetramer in solution. The BPs gp33103 repressor binds to five regulatory regions spanning the BPs genome, and regulates four promoters including the early lytic promoter, PR. BPs gp33103 has a complex pattern of DNA reco...Continue Reading

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Citations

Apr 27, 2016·Journal of Molecular Biology·Bryce L Lunt, Graham F Hatfull
Jun 6, 2019·MBio·Travis N Mavrich, Graham F Hatfull
May 6, 2016·Molecular Microbiology·Rebekah M DedrickGraham F Hatfull
Sep 30, 2020·Annual Review of Virology·Graham F Hatfull

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Methods Mentioned

BETA
Electrophoretic
PCR
footprinting
electrophoresis
RNAseq
gel filtration

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