An Updated Perspective on Sinorhizobium meliloti Chemotaxis to Alfalfa Flavonoids

Frontiers in Microbiology
K Karl ComptonBirgit E Scharf

Abstract

The symbiotic interaction between leguminous plants and their cognate rhizobia allows for the fixation of gaseous dinitrogen into bioavailable ammonia. The perception of host-derived flavonoids is a key initial step for the signaling events that must occur preceding the formation of the nitrogen-fixing organ. Past work investigating chemotaxis - the directed movement of bacteria through chemical gradients - of Bradyrhizobium japonicum, Rhizobium leguminosarum, and Rhizobium meliloti discovered chemotaxis to various organic compounds, but focused on chemotaxis to flavonoids because of their relevance to the symbiosis biochemistry. The current work sought to replicate and further examine Sinorhizobium (Ensifer) meliloti chemotaxis to the flavonoids previously thought to act as the principal attractant molecules prior to the initial signaling stage. Exudate from germinating alfalfa seedlings was analyzed for composition and quantities of different flavonoid compounds using mass spectrometry. The abundance of four prevalent flavonoids in germinating alfalfa seed exudates (SEs) was at a ratio of 200:5:5:1 for hyperoside, luteolin, luteolin-7-glucoside, and chrysoeriol. Using quantitative chemotaxis capillary assays, we did not detec...Continue Reading

References

Jun 25, 1992·Nature·R F Fisher, S R Long
Sep 1, 1991·Applied and Environmental Microbiology·W M BarbourG Stacey
Jan 1, 1991·Annual Review of Cell Biology·N J Brewin
Mar 1, 1990·Applied and Environmental Microbiology·V TorsvikF L Daae
Oct 27, 1972·Nature·R P Futrelle, H C Berg
Feb 1, 1980·Journal of Bacteriology·P AmesK Bergman
Feb 1, 1980·Journal of Bacteriology·C Napoli, P Albersheim
Nov 1, 1996·Molecular Microbiology·V Sourjik, R Schmitt
Jan 27, 2000·Phytochemistry·G A WächterB N Timmermann
Dec 26, 2002·Annual Review of Biochemistry·Howard C Berg
Apr 1, 1992·Applied and Environmental Microbiology·A J Dharmatilake, W D Bauer
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ady Vaknin, Howard C Berg
May 1, 1976·Plant Physiology·W W Currier, G A Strobel
Aug 1, 1990·Plant Physiology·C A Maxwell, D A Phillips
Jul 21, 2006·Journal of Bacteriology·Melicent C PeckSharon R Long
Sep 19, 2006·Journal of Bacteriology·Christine RotterBirgit Scharf
Dec 26, 2006·Journal of Bacteriology·Veronika M MeierBirgit E Scharf
Jun 27, 2007·Trends in Plant Science·Senthil SubramanianOliver Yu
Nov 29, 2007·The ISME Journal·Luiz F W RoeschEric W Triplett
Jul 21, 2009·Journal of Bacteriology·Veronika M Meier, Birgit E Scharf
Aug 16, 2011·Annual Review of Genetics·Giles E D OldroydJ Allan Downie
Oct 1, 2011·Current Biology : CB·R Ford Denison, E Toby Kiers
Dec 16, 2011·Cellular Microbiology·Dong WangHongyan Zhu
Sep 25, 2012·FEMS Microbiology Reviews·Andreas F HaagGail P Ferguson
Mar 5, 2013·Annual Review of Plant Biology·Michael Udvardi, Philip S Poole
Feb 4, 2014·PloS One·Xavier Raynaud, Naoise Nunan
Mar 25, 2014·Applied and Environmental Microbiology·Benjamin A WebbBirgit E Scharf

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Citations

May 22, 2021·Frontiers in Plant Science·K Karl Compton, Birgit E Scharf
Jun 25, 2021·Frontiers in Plant Science·Soo In SohnYoun Sung Cho
Jun 26, 2021·Plant Physiology and Biochemistry : PPB·Marco Antônio BosseAndré Rodrigues Dos Reis
Jul 10, 2021·Frontiers in Plant Science·María J SotoPieter van Dillewijn
Jul 7, 2021·FEMS Microbiology Reviews·Remy ColinVictor Sourjik
Jun 12, 2021·Bioscience, Biotechnology, and Biochemistry·Akifumi Sugiyama

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BETA
metabolomics profiling

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MassLynx
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