PMID: 7541131Jun 11, 1995Paper

An upstream U-snRNA gene-like promoter is required for transcription of the Arabidopsis thaliana 7SL RNA gene

Nucleic Acids Research
D J HeardJ M Gualberto

Abstract

The genes transcribed by RNA polymerase (pol) III can be placed into four distinct groups based on the nature and position of their promoter elements. In the higher eukaryotes equivalent genes usually belong to the same sub-type of pol III promoters and there are few examples of genes which have changed promoter type during evolution. In this work we demonstrate that the promoter of the Arabidopsis thaliana 7SL RNA gene is located upstream of the coding region and is identical to the promoters of pol III-specific plant U-small nuclear RNA (U-snRNA) genes. Sequence analysis of two different 7SL genes from A. thaliana revealed that both genes contain two sequence elements in their 5' flanking regions identical in sequence and position to the highly conserved USE and TATA elements of the pol III-transcribed plant U-snRNA genes. Mutational analysis of these elements in the At7SL-2 gene indicates that the USE and TATA elements are both necessary and account for > or = 90% of the transcriptional activity of this gene in transfected plant protoplasts. Within the coding region of both genes there is a sequence element which is a 10/11 nt match to the consensus B-box element of tRNA genes, however, this element is not important for gene...Continue Reading

References

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Citations

Dec 16, 2006·Current Genetics·Andrea Hinas, Fredrik Söderbom
Jul 6, 2001·The Plant Journal : for Cell and Molecular Biology·P ArnaudJ M Deragon
Jul 26, 2002·Nucleic Acids Research·Marco RegaliaTore Samuelsson
Aug 31, 2004·Nucleic Acids Research·Anders AspegrenFredrik Söderbom
Jun 18, 2005·The Plant Journal : for Cell and Molecular Biology·Yasushi YukawaMasahiro Sugiura
Aug 16, 2005·The Plant Journal : for Cell and Molecular Biology·Misato Ohtani, Munetaka Sugiyama
Dec 11, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J Müller, B J Benecke

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