Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies.

The Journal of Cell Biology
Naomi R StevensJordan W Raff

Abstract

Recent studies have identified a conserved "core" of proteins that are required for centriole duplication. A small number of additional proteins have recently been identified as potential duplication factors, but it is unclear whether any of these proteins are components of the core duplication machinery. In this study, we investigate the function of one of these proteins, Drosophila melanogaster Ana3. We show that Ana3 is present in centrioles and basal bodies, but its behavior is distinct from that of the core duplication proteins. Most importantly, we find that Ana3 is required for the structural integrity of both centrioles and basal bodies and for centriole cohesion, but it is not essential for centriole duplication. We show that Ana3 has a mammalian homologue, Rotatin, that also localizes to centrioles and basal bodies and appears to be essential for cilia function. Thus, Ana3 defines a conserved family of centriolar proteins and plays an important part in ensuring the structural integrity of centrioles and basal bodies.

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Citations

Nov 28, 2013·PloS One·David FournierMiguel A Andrade-Navarro
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hisham Bazzi, Kathryn V Anderson
Feb 4, 2010·The Journal of Cell Biology·Naomi R StevensJordan W Raff
Dec 4, 2013·The Journal of Cell Biology·Anna FranzJordan W Raff
Nov 27, 2015·American Journal of Human Genetics·Hanan ShamseldinFowzan S Alkuraya
Sep 8, 2010·The EMBO Journal·Hannah MüllerBodo M H Lange
Dec 15, 2010·Current Biology : CB·Paul T ConduitJordan W Raff
Dec 15, 2010·Developmental Cell·Naomi R StevensJordan W Raff
Mar 18, 2019·Brain : a Journal of Neurology·Laura V VandervoreGrazia M S Mancini
Jun 17, 2020·The Journal of Cell Biology·Pallavi PandaDavid M Glover
May 12, 2020·Molecular Biology and Evolution·Alexandre M Harris, Michael DeGiorgio
Apr 5, 2019·Brain : a Journal of Neurology·Ana Uzquiano, Fiona Francis
Feb 4, 2021·The Journal of Cell Biology·Yuan TianJingyan Fu
May 21, 2021·Saudi Journal of Biological Sciences·Muhammad Imran NaseerAdeel G Chaudhary
Jun 23, 2021·Journal of Cell Science·Ines Alvarez-RodrigoJordan W Raff
Sep 4, 2012·American Journal of Human Genetics·Sima Kheradmand KiaGrazia M S Mancini
Jan 9, 2022·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Yuan TianJingyan Fu

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Methods Mentioned

BETA
transgenic
transfection

Software Mentioned

ClustalW2
BLAST
Jalview
Photoshop
Gatan
DigitalMicrograph
Volocity
Adobe
UltraView ERS
BestGene

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