Anabaena apoflavodoxin hydrogen exchange: on the stable exchange core of the alpha/beta(21345) flavodoxin-like family

Proteins
G M LangdonM Rico

Abstract

An important issue in modern protein biophysics is whether structurally homologous proteins share common stability and/or folding features. Flavodoxin is an archetypal alpha/beta protein organized in three layers: a central beta-sheet (strand order 21345) flanked by helices 1 and 5 on one side and helices 2, 3, and 4 on the opposite side. The backbone internal dynamics of the apoflavodoxin from Anabaena is analyzed here by the hydrogen exchange method. The hydrogen exchange rates indicate that 46 amide protons, distributed throughout the structure of apoflavodoxin, exchange relatively slowly at pH 7.0 (k(ex) < 10(-1) min(-1)). According to their distribution in the structure, protein stability is highest on the beta-sheet, helix 4, and on the layer formed by helices 1 and 5. The exchange kinetics of Anabaena apoflavodoxin was compared with those of the apoflavodoxin from Azotobacter, with which it shares a 48% sequence identity, and with Che Y and cutinase, two other alpha/beta (21345) proteins with no significant sequence homology with flavodoxins. Both similarities and differences are observed in the cores of these proteins. It is of interest that a cluster of a few structurally equivalent residues in the central beta-strands...Continue Reading

References

Nov 1, 1992·Protein Science : a Publication of the Protein Society·S T RaoM Sundarlingam
Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Sep 8, 1965·Biochemical and Biophysical Research Communications·R M Smillie
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Nov 1, 1983·Quarterly Reviews of Biophysics·S W Englander, N R Kallenbach
Apr 25, 1983·Journal of Molecular Biology·W W SmithK T Yasunobu
Jun 1, 1994·Nature Structural Biology·V Muñoz, L Serrano
Sep 1, 1994·Proteins·Y BaiS W Englander
Sep 1, 1993·Proteins·Y BaiS W Englander
Oct 1, 1993·Trends in Biochemical Sciences·C Woodward
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 1, 1996·Current Opinion in Structural Biology·S W EnglanderL Mayne
Jul 1, 1996·Protein Science : a Publication of the Protein Society·C G GenzorJ Sancho
Aug 1, 1997·Trends in Biochemical Sciences·J ClarkeA R Fersht
Feb 12, 1998·Protein Science : a Publication of the Protein Society·J J PrompersC W Hilbers
Aug 7, 1998·Biochemistry·S MaldonadoJ Sancho
Aug 12, 1998·Nature Structural Biology·J C MartinezL Serrano
Nov 12, 1998·Trends in Biochemical Sciences·S W Englander
Nov 12, 1998·Trends in Biochemical Sciences·C Woodward, R Li
Nov 25, 1998·Protein Science : a Publication of the Protein Society·C P van MierloE Steensma
Jul 2, 1999·Journal of Molecular Biology·J Fernández-RecioJ Sancho
Aug 19, 1999·Protein Science : a Publication of the Protein Society·R Li, C Woodward
Mar 29, 2000·The Journal of Biological Chemistry·A LostaoJ Sancho
Mar 13, 2001·Journal of Molecular Biology·M P IrúnJ Sancho
Oct 1, 1955·Biochimica Et Biophysica Acta·A HVIDT
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

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Citations

Sep 14, 2011·Biochemistry·S Ayuso-TejedorJ Sancho
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Megan GuelkerYousif Shamoo
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Susana MaldonadoJavier Sancho
Aug 29, 2012·PLoS Computational Biology·Rebeca García-FandiñoModesto Orozco
Oct 31, 2012·PloS One·Vladimir Espinosa Angarica, Javier Sancho
Sep 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthew AutonD Wayne Bolen
Aug 1, 2006·Protein Science : a Publication of the Protein Society·Marta BuenoJavier Sancho
Mar 28, 2006·Journal of Molecular Biology·Marta BuenoJavier Sancho
Jun 18, 2010·Journal of Molecular Biology·Sara Ayuso-TejedorM Angeles Jiménez
Sep 18, 2012·Biochimica Et Biophysica Acta·Isaias LansMilagros Medina
Apr 6, 2017·The FEBS Journal·Joseline A Houwman, Carlo P M van Mierlo
Aug 20, 2004·The Journal of Biological Chemistry·Jon López-LlanoJavier Sancho
Apr 19, 2003·The Journal of Biological Chemistry·Anabel LostaoJavier Sancho
Feb 10, 2021·Nature Communications·Rishav MitraJames C A Bardwell

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