Anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium Pseudomonas alcaligenes strain DIP1 and role of three other coisolates in a mixed bacterial consortium.

Applied and Environmental Microbiology
Ahmed Sallam, Alexander Steinbüchel

Abstract

Four bacterial strains were isolated from a cyanophycin granule polypeptide (CGP)-degrading anaerobic consortium, identified by 16S rRNA gene sequencing, and assigned to species of the genera Pseudomonas, Enterococcus, Clostridium, and Paenibacillus. The consortium member responsible for CGP degradation was assigned as Pseudomonas alcaligenes strain DIP1. The growth of and CGP degradation by strain DIP1 under anaerobic conditions were enhanced but not dependent on the presence of nitrate as an electron acceptor. CGP was hydrolyzed to its constituting beta-Asp-Arg dipeptides, which were then completely utilized within 25 and 4 days under anaerobic and aerobic conditions, respectively. The end products of CGP degradation by strain DIP1 were alanine, succinate, and ornithine as determined by high-performance liquid chromatography analysis. The facultative anaerobic Enterococcus casseliflavus strain ELS3 and the strictly anaerobic Clostridium sulfidogenes strain SGB2 were coisolates and utilized the beta-linked isodipeptides from the common pool available to the mixed consortium, while the fourth isolate, Paenibacillus odorifer strain PNF4, did not play a direct role in the biodegradation of CGP. Several syntrophic interactions aff...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1987·Methods in Enzymology·R N RaoS Kuhstoss
Sep 1, 1986·Microbiological Reviews·R CuninV Stalon
Jan 1, 1971·Annual Review of Biochemistry·A J Sussman, C Gilvarg
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jun 1, 1983·Journal of Bacteriology·M M Allen, M A Hawley
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Jul 2, 1998·Applied and Environmental Microbiology·G GerritseW J Quax
Jul 30, 1999·International Journal of Systematic Bacteriology·W AchouakT Heulin
Jan 11, 2000·Archives of Microbiology·E AboulmagdA Steinbüchel
Feb 24, 2001·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·D P StephanE K Pistorius
Mar 28, 2002·Applied and Environmental Microbiology·Lauren L WingardA Michelle Wood
May 4, 2002·The Biochemical Journal·Mahdi HejaziWolfgang Lockau
Jun 29, 2002·Applied and Environmental Microbiology·Kay M FreyAlexander Steinbüchel
Aug 2, 2003·International Journal of Systematic and Evolutionary Microbiology·A P UetanabaroD Fritze
Jul 13, 2004·Biomacromolecules·Martin Obst, Alexander Steinbüchel
Feb 5, 2005·Applied and Environmental Microbiology·Yasser ElbahloulAlexander Steinbüchel
Dec 8, 2005·Applied and Environmental Microbiology·Yasser ElbahloulAlexander Steinbüchel
Jun 1, 1986·Applied and Environmental Microbiology·G E JennemanR M Knapp
Mar 28, 2007·Applied Microbiology and Biotechnology·Elinor ScottJohan Sanders
Sep 19, 2007·Applied Microbiology and Biotechnology·Hans MooibroekAlexander Steinbüchel

❮ Previous
Next ❯

Citations

Dec 12, 2012·Applied Microbiology and Biotechnology·Katja AdamesAlexander Steinbüchel
Nov 4, 2008·Applied and Environmental Microbiology·Ahmed SallamAlexander Steinbüchel
Jul 14, 2009·Journal of Molecular Biology·Adrienne M LawMatthew S Kimber
Jun 23, 2009·International Journal of Systematic and Evolutionary Microbiology·Ahmed Sallam, Alexander Steinbüchel
Jun 21, 2017·International Journal of Systematic and Evolutionary Microbiology·Anastasiya ZakharyukViktoria Shcherbakova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.