PMID: 8580644Jan 1, 1995Paper

Anaerobic dechlorination of trichloroethene, tetrachloroethene and 1,2-dichloroethane by an acetogenic mixed culture in a fixed-bed reactor

Biodegradation
A P WildT Leisinger

Abstract

An anaerobic enrichment culture with glucose as the sole source of carbon and energy plus trichloroethene (TCE) as a potential electron acceptor was inoculated with material from a full size anaerobic charcoal reactor that biologically eliminated dichloromethane from contaminated groundwater (Stromeyer et al. 1991). In subcultures of this enrichment complete sequential transformation of 10 microM TCE via cis-dichloroethene and chloroethene to ethene was reproducibly observed. Maintenance of this activity on subcultivation required the presence of TCE in the medium. The enrichment culture was used to inoculate an anaerobic fixed-bed reactor containing sintered glass Raschig elements as support material. The reactor had a total volume of 1780 ml and was operated at 20 degrees C in an up-flow mode with a flow rate of 50 ml/h. It was fed continuously with 2 mM glucose and 55 microM TCE. Glucose was converted to acetate as the major product and to a minor amount of methane; TCE was quantitatively dehalogenated to ethene. When, in addition to TCE, tetrachloroethene or 1,2-dichloroethane were added to the system, these compounds were also dehalogenated to ethene. In contrast, 1,1,1-trichloroethane was not dehalogenated, but at 40 micr...Continue Reading

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Citations

Feb 9, 2000·Folia Microbiologica·J Damborský
Jun 1, 1996·Current Opinion in Biotechnology·T Leisinger
Sep 6, 2003·Applied and Environmental Microbiology·Stefaan De WildemanWilly Verstraete
Jan 19, 1999·Annual Review of Microbiology·M D LeeR J Buchanan
Jan 10, 2014·Journal of Environmental Health Science & Engineering·Sina DobaradaranTorsten C Schmidt
Jan 30, 1999·Applied and Environmental Microbiology·C B MiguezD Groleau

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