Abstract
An anaerobic enrichment culture with glucose as the sole source of carbon and energy plus trichloroethene (TCE) as a potential electron acceptor was inoculated with material from a full size anaerobic charcoal reactor that biologically eliminated dichloromethane from contaminated groundwater (Stromeyer et al. 1991). In subcultures of this enrichment complete sequential transformation of 10 microM TCE via cis-dichloroethene and chloroethene to ethene was reproducibly observed. Maintenance of this activity on subcultivation required the presence of TCE in the medium. The enrichment culture was used to inoculate an anaerobic fixed-bed reactor containing sintered glass Raschig elements as support material. The reactor had a total volume of 1780 ml and was operated at 20 degrees C in an up-flow mode with a flow rate of 50 ml/h. It was fed continuously with 2 mM glucose and 55 microM TCE. Glucose was converted to acetate as the major product and to a minor amount of methane; TCE was quantitatively dehalogenated to ethene. When, in addition to TCE, tetrachloroethene or 1,2-dichloroethane were added to the system, these compounds were also dehalogenated to ethene. In contrast, 1,1,1-trichloroethane was not dehalogenated, but at 40 micr...Continue Reading
References
Apr 1, 1977·Mutation Research·M M Shahin, R C Von Borstel
Jan 1, 1991·Biodegradation·S A StromeyerT Leisinger
Jan 1, 1990·Biodegradation·C HolligerA J Zehnder
Jun 1, 1992·Applied and Environmental Microbiology·W P de BruinA J Zehnder
Jul 1, 1992·Journal of Bacteriology·C HolligerA J Zehnder
Jan 1, 1991·Annual Review of Microbiology·B D Ensley
Aug 1, 1991·Applied and Environmental Microbiology·T D DiStefanoS H Zinder
Jun 1, 1991·Applied and Environmental Microbiology·B R Folsom, P J Chapman
Sep 1, 1989·Applied and Environmental Microbiology·D L Freedman, J M Gossett
Apr 1, 1989·Applied and Environmental Microbiology·R Gälli, P L McCarty
Jul 1, 1981·Archives of Microbiology·F Widdel, N Pfennig
Jul 1, 1994·Journal of Applied Toxicology : JAT·M D Easter, R Von Burg
Sep 1, 1993·Applied and Environmental Microbiology·C HolligerA J Zehnder
Jul 1, 1993·Applied and Environmental Microbiology·G D HopkinsP L McCarty
Jul 1, 1987·Applied and Environmental Microbiology·N Belay, L Daniels
Jan 1, 1994·Applied and Environmental Microbiology·B D Ensley, P R Kurisko
Apr 5, 1992·Biotechnology and Bioengineering·J J Heijnen, J P Van Dijken
Sep 20, 1993·Biotechnology and Bioengineering·D E FennellW J Jewell
Citations
Feb 9, 2000·Folia Microbiologica·J Damborský
May 23, 2006·Biodegradation·Federico AulentaValter Tandoi
Nov 8, 2002·Water Research·Melanie DuhamelElizabeth A Edwards
Jun 1, 1996·Current Opinion in Biotechnology·T Leisinger
Oct 20, 2005·Journal of Bioscience and Bioengineering·T LeeK Furukawa
Mar 10, 2009·Applied and Environmental Microbiology·Ariel Grostern, Elizabeth A Edwards
Dec 4, 2012·Applied and Environmental Microbiology·Chang DingJianzhong He
Sep 6, 2003·Applied and Environmental Microbiology·Stefaan De WildemanWilly Verstraete
Dec 3, 2004·Applied and Environmental Microbiology·Tamzen W MacbethKent S Sorenson
Jan 19, 1999·Annual Review of Microbiology·M D LeeR J Buchanan
Sep 18, 2007·International Journal of Hygiene and Environmental Health·Thomas KistemannHarald Färber
Jan 22, 2005·Journal of Applied Microbiology·O TresseS Guiot
Apr 24, 2008·Biotechnology and Bioengineering·Simona RossettiValter Tandoi
Jan 10, 2014·Journal of Environmental Health Science & Engineering·Sina DobaradaranTorsten C Schmidt
Nov 26, 2013·Journal of Contaminant Hydrology·Kinga M RévészEurybiades Busenberg
Jul 15, 2015·Biodegradation·Michelle M LorahDuane Graves
Jan 1, 1996·Biodegradation·A WildT Leisinger
Jan 30, 1999·Applied and Environmental Microbiology·C B MiguezD Groleau