Anaerobic decomposition of switchgrass by tropical soil-derived feedstock-adapted consortia.

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Kristen M DeAngelisTerry C Hazen

Abstract

Tropical forest soils decompose litter rapidly with frequent episodes of anoxic conditions, making it likely that bacteria using alternate terminal electron acceptors (TEAs) play a large role in decomposition. This makes these soils useful templates for improving biofuel production. To investigate how TEAs affect decomposition, we cultivated feedstock-adapted consortia (FACs) derived from two tropical forest soils collected from the ends of a rainfall gradient: organic matter-rich tropical cloud forest (CF) soils, which experience sustained low redox, and iron-rich tropical rain forest (RF) soils, which experience rapidly fluctuating redox. Communities were anaerobically passed through three transfers of 10 weeks each with switchgrass as a sole carbon (C) source; FACs were then amended with nitrate, sulfate, or iron oxide. C mineralization and cellulase activities were higher in CF-FACs than in RF-FACs. Pyrosequencing of the small-subunit rRNA revealed members of the Firmicutes, Bacteroidetes, and Alphaproteobacteria as dominant. RF- and CF-FAC communities were not different in microbial diversity or biomass. The RF-FACs, derived from fluctuating redox soils, were the most responsive to the addition of TEAs, while the CF-FACs w...Continue Reading

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Citations

Jan 21, 2014·Journal of Agricultural and Food Chemistry·Antonius G M LeijdekkersHenk A Schols
Feb 28, 2014·Environmental Science & Technology·Michael R WeigandJorge W Santo Domingo
Feb 16, 2016·Microbial Biotechnology·Elisa KorenblumJan Dirk van Elsas
Jan 10, 2018·Frontiers in Microbiology·Lucas AuerGuillermina Hernandez-Raquet

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Methods Mentioned

BETA
PCR

Software Mentioned

PyroTagger

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