Anaerobic degradation of benzene by a marine sulfate-reducing enrichment culture, and cell hybridization of the dominant phylotype

Environmental Microbiology
Florin Musat, Friedrich Widdel

Abstract

The anaerobic biodegradation of benzene, a common constituent of petroleum and one of the least reactive aromatic hydrocarbons, is insufficiently understood with respect to the involved microorganisms and their metabolism. To study these aspects, sulfate-reducing bacteria were enriched with benzene as sole organic substrate using marine sediment as inoculum. Repeated subcultivation yielded a sediment-free enrichment culture constituted of mostly oval-shaped cells and showing benzene-dependent sulfate reduction and growth under strictly anoxic conditions. Amplification and sequencing of 16S rRNA genes from progressively diluted culture samples revealed an abundant phylotype; this was closely related to a clade of Deltaproteobacteria that includes sulfate-reducing bacteria able to degrade naphthalene or other aromatic hydrocarbons. Cell hybridization with two specifically designed 16S rRNA-targeted fluorescent oligonucleotide probes showed that the retrieved phylotype accounted for more than 85% of the cells detectable via DAPI staining (general cell staining) in the enrichment culture. The result suggests that the detected dominant phylotype is the 'candidate species' responsible for the anaerobic degradation of benzene. Quantit...Continue Reading

References

Feb 1, 1987·Applied and Environmental Microbiology·D Grbić-Galić, T M Vogel
May 1, 1993·Applied and Environmental Microbiology·R RabusF Widdel
Feb 1, 1996·Applied and Environmental Microbiology·J ZhouJ M Tiedje
Dec 15, 1996·FEMS Microbiology Letters·C D PhelpsL Y Young
Jul 1, 1997·Applied and Environmental Microbiology·J SnaidrK H Schleifer
Jan 30, 1999·Applied and Environmental Microbiology·S M Burland, E A Edwards
Jul 2, 1999·Applied and Environmental Microbiology·J N Rooney-VargaD R Lovley
Jul 6, 2000·Applied and Environmental Microbiology·R U MeckenstockB Schink
Aug 5, 2000·Journal of Molecular Biology·I T PaulsenM H Saier
Jul 7, 2001·Biodegradation·D R Lovley
Feb 1, 2003·Research in Microbiology·John D CoatesMichael J McInerney
Feb 1, 2003·Environmental Microbiology·Ania C Ulrich, Elizabeth A Edwards
Feb 7, 2003·Applied and Environmental Microbiology·Olaf KniemeyerFriedrich Widdel
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
May 6, 2004·Archives of Microbiology·Barbara MoraschRainer U Meckenstock
Sep 30, 2005·Environmental Science & Technology·Ania C UlrichElizabeth A Edwards
Jan 21, 2006·Environmental Microbiology·Michael Safinowski, Rainer U Meckenstock
Mar 1, 1995·Applied and Environmental Microbiology·D R LovleyE Phillips
Mar 14, 2007·Current Opinion in Chemical Biology·Johann Heider
Nov 29, 2007·The ISME Journal·Irene A DavidovaJoseph M Suflita

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Citations

May 24, 2011·Applied and Environmental Microbiology·Kevin KuntzeMatthias Boll
Jul 12, 2011·Applied and Environmental Microbiology·Dawn E HolmesDerek R Lovley
Oct 8, 2013·Applied and Environmental Microbiology·Tian ZhangDerek R Lovley
Feb 24, 2016·Frontiers in Microbiology·Thomas AülloAnthony Ranchou-Peyruse
Dec 5, 2009·FEMS Microbiology Ecology·Amelia-Elena RotaruJens Harder
Jan 7, 2015·Biotechnology Advances·Wen-Wei Li, Han-Qing Yu
May 12, 2009·Advances in Applied Microbiology·Larry L Barton, Guy D Fauque
Dec 12, 2020·Frontiers in Microbiology·Krisztián LacziKatalin Perei
May 28, 2021·Environmental Science & Technology·Courtney R A TothElizabeth A Edwards

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