Abstract
The anaerobic degradation of tetradecylamine and other long-chain alkylamines by a newly isolated denitrifying bacterium was studied. Strain ZN6 was isolated from a mixture of soil and active sludge and was identified as representing Pseudomonas stutzeri, based on partial 16S rRNA gene sequence analysis. Strain ZN6 was a mesophilic, motile, Gram-negative rod-shaped bacterium and was able to grow on a variety of compounds including even-numbered primary fatty amines with alkyl chains ranging from C(4) to C(18) coupled to nitrate reduction. Alkylamines were used as sole carbon, energy and nitrogen source and were completely mineralized. Nitrate was dissimilated by ZN6 to nitrite. When strain ZN6 was grown under nitrate limitation, nitrite was slowly dissimilated further. When cocultivated with the complete denitrifier Castellaniella defragens ZN3, anaerobic degradation under denitrifying of alkylamines by strain ZN6 was slightly faster. Strain ZN3 is a complete denitrifier, unable to convert tetradecylamine, and was copurified from the same enrichment culture as strain ZN6. The proposed pathway for the degradation of alkylamines in strain ZN6 starts with C-N cleavages to alkanals and further oxidation to the corresponding fatty a...Continue Reading
References
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1973·The Biochemical Journal·R E Cripps, A S Noble
Jan 1, 1973·The Biochemical Journal·J Colby, L J Zatman
Jul 1, 1982·The Biochemical Journal·I V FryE J Hewitt
Oct 1, 1994·Applied Microbiology and Biotechnology·A G KroonC G van Ginkel
Nov 1, 1995·Antonie van Leeuwenhoek·J C Scholten, A J Stams
Jun 8, 2000·International Journal of Systematic and Evolutionary Microbiology·C M PluggeA J Stams
Jul 6, 2000·Systematic and Applied Microbiology·R Petri, J F Imhoff
Nov 25, 2000·Biochimica Et Biophysica Acta·M J Scott, M N Jones
May 30, 2003·Applied Microbiology and Biotechnology·J A DijkJ Gerritse
Sep 1, 1957·Bacteriological Reviews·W VISHNIAC, M SANTER
Feb 6, 2004·Current Microbiology·Seung-Hun BaekSung-Taik Lee
Jul 6, 2004·Archives of Biochemistry and Biophysics·Victor L Davidson
Oct 27, 2004·Archives of Microbiology·John A DijkAlfons J M Stams
Jan 26, 2005·Biochemical Society Transactions·K T Van de Pas-SchoonenM S M Jetten
Aug 1, 1985·Applied and Environmental Microbiology·D J Burdige, K H Nealson
Apr 1, 1986·Applied and Environmental Microbiology·D R Lovley, E J Phillips