Anaerobic digestion of renewable biomass: thermophilic temperature governs methanogen population dynamics.

Applied and Environmental Microbiology
Niclas KrakatP Scherer

Abstract

Beet silage and beet juice were digested continuously as representative energy crops in a thermophilic biogas fermentor for more than 7 years. Fluorescence microscopy of 15 samples covering a period of 650 days revealed that a decrease in temperature from 60 degrees C to 55 degrees C converted a morphologically uniform archaeal population (rods) into a population of methanogens exhibiting different cellular morphologies (rods and coccoid cells). A subsequent temperature increase back to 60 degrees C reestablished the uniform morphology of methanogens observed in the previous 60 degrees C period. In order to verify these observations, representative samples were investigated by amplified rRNA gene restriction analysis (ARDRA) and fluorescence in situ hybridization (FISH). Both methods confirmed the temperature-dependent population shift observed by fluorescence microscopy. Moreover, all samples investigated demonstrated that hydrogenotrophic Methanobacteriales dominated in the fermentor, as 29 of 34 identified operational taxonomic units (OTUs) were assigned to this order. This apparent discrimination of acetoclastic methanogens contradicts common models for anaerobic digestion processes, such as anaerobic digestion model 1 (ADM...Continue Reading

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Citations

Nov 10, 2010·Applied Microbiology and Biotechnology·Ayrat M ZiganshinSabine Kleinsteuber
Jul 9, 2013·Waste Management·Sophia A GhanimehMutasem El-Fadel
Aug 3, 2010·Applied and Environmental Microbiology·Niclas KrakatPaul Scherer
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Jun 26, 2020·Frontiers in Microbiology·D'Arcy R Meyer-DombardJudy Malas

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