Anaerobic Growth of Haloarchaeon Haloferax volcanii by Denitrification Is Controlled by the Transcription Regulator NarO

Journal of Bacteriology
Tatsuya HattoriTaketomo Fujiwara

Abstract

The extremely halophilic archaeon Haloferax volcanii grows anaerobically by denitrification. A putative DNA-binding protein, NarO, is encoded upstream of the respiratory nitrate reductase gene of H. volcanii. Disruption of the narO gene resulted in a loss of denitrifying growth of H. volcanii, and the expression of the recombinant NarO recovered the denitrification capacity. A novel CXnCXCX7C motif showing no remarkable similarities with known sequences was conserved in the N terminus of the NarO homologous proteins found in the haloarchaea. Restoration of the denitrifying growth was not achieved by expression of any mutant NarO in which any one of the four conserved cysteines was individually replaced by serine. A promoter assay experiment indicated that the narO gene was usually transcribed, regardless of whether it was cultivated under aerobic or anaerobic conditions. Transcription of the genes encoding the denitrifying enzymes nitrate reductase and nitrite reductase was activated under anaerobic conditions. A putative cis element was identified in the promoter sequence of haloarchaeal denitrifying genes. These results demonstrated a significant effect of NarO, probably due to its oxygen-sensing function, on the transcriptio...Continue Reading

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Citations

Nov 28, 2017·Annual Review of Genetics·Mar Martinez-PastorAmy K Schmid
Jun 13, 2018·FEMS Microbiology Reviews·Callum J D LeeJohn E Hallsworth
Nov 2, 2016·Frontiers in Microbiology·Eva C PreisnerRobert S Norman
Mar 23, 2017·Extremophiles : Life Under Extreme Conditions·Volkan KilicRosa María Martínez-Espinosa
Jun 18, 2020·International Journal of Molecular Sciences·Rosa María Martínez-Espinosa
Dec 23, 2017·FEMS Microbiology Letters·Hannah GaimsterGary Rowley
Mar 21, 2020·Extremophiles : Life Under Extreme Conditions·Jing HouHeng-Lin Cui
Jan 19, 2021·Microbiology·Roshali T de SilvaIain G Duggin

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