Anaerobic methyl tert-butyl ether-degrading microorganisms identified in wastewater treatment plant samples by stable isotope probing.

Applied and Environmental Microbiology
Weimin SunAlison M Cupples

Abstract

Anaerobic methyl tert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isotope probing (SIP) targeted to both bacteria and archaea to identify the putative MTBE degraders. For this purpose, DNA was extracted at two time points, subjected to ultracentrifugation, fractioning, and terminal restriction fragment length polymorphism (TRFLP). In addition, bacterial and archaeal 16S rRNA gene clone libraries were constructed. The SIP experiments indicated bacteria in the phyla Firmicutes (family Ruminococcaceae) and Alphaproteobacteria (genus Sphingopyxis) were the dominant MTBE degraders. Previous studies have suggested a role for Firmicutes in anaerobic MTBE degradation; however, the putative MTBE-degrading microorganism in the current study is a novel MTBE-degrading phylotype within this phylum. Two archaeal phylotypes (genera Methanosarcina and Methanocorpusculum) were also enriched in the heavy fractions, and these organisms may be responsible for minor amounts of MTBE degradation or for the...Continue Reading

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Citations

Oct 31, 2015·International Journal of Environmental Research and Public Health·Hai-Han ZhangWei-Xing Ma
May 20, 2015·Applied Microbiology and Biotechnology·Fernanda PaesAlison M Cupples
Feb 28, 2015·Environmental Science and Pollution Research International·Indumathy Jayamani, Alison M Cupples
Sep 23, 2014·Applied Microbiology and Biotechnology·Yuyin YangYi Huang
Mar 11, 2020·International Journal of Environmental Research and Public Health·Sheng-Nan ChenMan-Man Du
Oct 1, 2013·Ground Water Monitoring & Remediation·Katherine C KeyDora Ogles
Mar 8, 2020·Journal of Hazardous Materials·S F ThorntonM J Spence
Jul 2, 2021·The Science of the Total Environment·Hongyu Dang, Alison M Cupples

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