Abstract
A novel thermoalkaliphilic, obligately anaerobic bacterium was isolated from a humid soil sample of a hot inlet of Lake Bogoria, Kenya. The newly isolated strain grows optimally at pH 9.5 and 50-55 degrees C and its growth range is pH 6.0-10.5 and 30-65 degrees C. Unlike the already known thermoalkaliphiles, the strain grows heterotrophically on a variety of mono- and polysaccharides (glucose, ribose, mannose, fructose, sucrose, maltose, starch, pullulan, xylan and cellulose) and on proteinaceous substrates such as yeast extract, peptone and tryptone. No dissimilatory sulfate reduction was observed, whereas thiosulfate was found to enhance growth when glucose or starch were used as substrates. Under optimal conditions, the doubling time is 48 min. Sodium ions are necessary for growth, with an optimal concentration of 230 mM (1% NaCl, w/v) at pH 9.5. The rod-shaped cells are motile in the exponential growth phase under optimal growth conditions. Despite the Gram-negative staining and negative KOH assay, the strain is a Gram-positive organism, having an atypically thin cell wall. A sheath-like structure occurs at the cell separation area and parts of a surface layer-like structure were also observed. Based on physiological proper...Continue Reading
Citations
Jul 27, 2007·Critical Reviews in Microbiology·Elena V PikutaJane Tang
Nov 13, 2015·Water Research·Xu WangJiuhui Qu
Apr 2, 2008·Annals of the New York Academy of Sciences·Isaac D Wagner, Juergen Wiegel
Jun 5, 2008·International Journal of Systematic and Evolutionary Microbiology·Vadim KevbrinJuergen Wiegel
Mar 5, 2015·Microbial Biotechnology·Katharina PorschMarcell Nikolausz
Jun 18, 2016·Scientific Reports·Xiaohu DaiBin Dong
Apr 24, 2007·Advances in Applied Microbiology·Moh'd Salameh, Juergen Wiegel
Aug 27, 2016·Environmental Science and Pollution Research International·Peng YanYu Shen
Aug 3, 2002·Environmental Microbiology·Peter StougaardOle C Hansen
Jun 17, 2008·Applied Biochemistry and Biotechnology·Ivan PishtiyskiBoriana Zhekova
Jan 10, 2006·Microbial Ecology·Hailiang DongMatthew W Fields
Jan 13, 2006·Applied Microbiology and Biotechnology·Volker ThiemannGarabed Antranikian
Jun 29, 2013·Extremophiles : Life Under Extreme Conditions·Vadim KevbrinBoris Kuznetsov
Feb 7, 2003·Applied and Environmental Microbiology·Shaheen B HumayounJames T Hollibaugh
Jul 8, 2005·Applied and Environmental Microbiology·Helena GradisarRoman Jerala
Jun 9, 2004·Applied and Environmental Microbiology·Costanzo BertoldoWolfgang Liebl
Nov 4, 2008·Applied and Environmental Microbiology·Ilya V KublanovElizaveta A Bonch-Osmolovskaya
Jun 4, 2005·Applied and Environmental Microbiology·Gengxin ZhangChuanlun Zhang
Jul 8, 2005·Applied and Environmental Microbiology·Meike BallschmiterWolfgang Liebl
May 15, 2004·International Journal of Systematic and Evolutionary Microbiology·Vladimir GorlenkoKenneth Nealson
Feb 23, 2017·Frontiers in Microbiology·Kaitlin R RempfertAlexis S Templeton
May 17, 2017·Extremophiles : Life Under Extreme Conditions·Melvin F SiliakusServé W M Kengen
Jun 1, 2002·Current Opinion in Chemical Biology·Costanzo Bertoldo, Garabed Antranikian
May 19, 2021·International Journal of Systematic and Evolutionary Microbiology·Marianne QuéméneurAnne Postec
Nov 26, 2010·Environmental Science & Technology·Peng ZhangYuxiao Zhao