Analyses of binding sequences of the PhaR protein of Rhodobacter sphaeroides FJ1

FEMS Microbiology Letters
Min-En Chou, Mei Kwei Yang

Abstract

The phaC, phaP, phaR, and phaZ genes are involved in the synthesis, accumulation, and degradation of poly-beta-hydroxybutyrate (PHB). These genes encode the PHB synthase, phasin, regulatory protein, and PHB depolymerase, respectively, and are located in the same locus in the genome of Rhodobacter sphaeroides FJ1, a purple nonsulfur bacterium capable of producing PHB. We have previously found that the PhaR protein binds to the promoter regions of phaP, phaR, and phaZ and represses their expression. In this study, we determined that PhaR binds to an 11-bp palindromic sequence, 5'-CTGCN(3)GCAG-3', located at nucleotides -69 to -59 and -97 to -87 relative to the translation start site of phaP. Substitution of the three spacer nucleotides with any three or four nucleotides in this sequence had no effect on PhaR binding, but all other base deletions or substitutions in this sequence abolished its ability to bind PhaR both in vitro and in vivo. These results suggest that PhaR regulates the expression of phaP in R. sphaeroides FJ1.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1992·FEMS Microbiology Reviews·A SteinbüchelH Valentin
Apr 1, 1993·FEMS Microbiology Reviews·A SteinbüchelH Valentin
Nov 12, 1996·Biochemical and Biophysical Research Communications·S F WengY H Tseng
Mar 14, 2001·Journal of Biotechnology·B Kessler, B Witholt
Sep 6, 2002·Annual Review of Microbiology·Dieter Jendrossek, Rene Handrick

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