Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy

Bone
Atsuhiko HikitaTakeshi Imamura

Abstract

Bone modeling and remodeling are cellular events during which osteoblast lineage cells and osteoclasts interact. During these events, cells undergo drastic changes with time as they become differentiated. Their morphology, topology, and activity are affected by other cells and the extracellular matrices. Since the mechanisms underlying the cellular events of bone metabolism have not been elucidated, there is a need for systems to analyze these cellular networks and their microenvironments spatiotemporally at the cellular level. Here we report a novel in vitro system for reconstituting the bone cell network of osteoclasts, osteoblasts, and osteocytes in the mineralized nodule, allowing for observation of bone modeling and remodeling phenomena by 2-photon microscopy. Using this system, the change in morphology of osteoblasts from cuboidal to flat cells was observed and measured during the formation of mineralized nodules. Furthermore, the recruitment of osteoblasts to resorption pits and their replenishment by newly formed matrices were successfully observed, providing strong evidence for the coupling of bone resorption and bone formation at cellular level. During such remodeling cycle, flat osteoblasts that survived more than 7 ...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O W PetersenM J Bissell
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J Pfeilschifter, G R Mundy
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G A HowardD J Baylink
Apr 1, 1982·The Anatomical Record·P T Tran VanR Baron
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·V EvertsW Beertsen
Sep 14, 2002·Science·George H Patterson, Jennifer Lippincott-Schwartz
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark J MillerIan Parker
May 19, 2004·Calcified Tissue International·M T K MulariH K Väänänen
Aug 27, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kenneth E S PooleJonathan Reeve
Aug 8, 2006·Cell Metabolism·Chen ZhaoKoichi Matsuo
May 24, 2007·Tissue Engineering·Ravi DhurjatiErwin A Vogler
Dec 20, 2008·The American Journal of Pathology·Thomas Levin AndersenJean-Marie Delaisse
Jun 6, 2009·PloS One·Lasse EvensenJames B Lorens
Jan 22, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lynda F Bonewald
Sep 13, 2011·Nature Medicine·Jinhu XiongCharles A O'Brien
Sep 13, 2011·Nature Medicine·Tomoki NakashimaHiroshi Takayanagi
May 25, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sang Wan KimHenry M Kronenberg
Mar 27, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Masashi HonmaHiroshi Suzuki
Jun 4, 2013·Nature Cell Biology·Cyrus M GhajarMina J Bissell
Jun 12, 2013·The American Journal of Pathology·Thomas L AndersenJean-Marie Delaisse
Aug 3, 2013·The Journal of Clinical Investigation·Sunao TakeshitaKyoji Ikeda
Sep 12, 2013·Journal of Cellular Physiology·Venkatesh KrishnanAndrea M Mastro
Jan 15, 2014·The American Journal of Pathology·Helene B KristensenJean-Marie Delaisse

❮ Previous
Next ❯

Citations

Jan 10, 2017·Expert Opinion on Biological Therapy·Leonardo BandeiraJohn P Bilezikian
May 26, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Masanori KoideNobuyuki Udagawa
Jan 16, 2021·Current Osteoporosis Reports·Sana AnsariSandra Hofmann
Oct 31, 2021·Scientific Reports·Yoshiyuki MiyamotoAtsuhiko Hikita

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Proceedings of the Society for Experimental Biology and Medicine
J F SheaJ J Taylor
© 2021 Meta ULC. All rights reserved