PMID: 8612604Mar 1, 1996Paper

Analyses of cis-acting and trans-acting elements that are crucial to sustain pregnancy-specific glycoprotein gene expression in different cell types

European Journal of Biochemistry
Nicolás P KoritschonerL C Patrito

Abstract

Pregnancy-specific beta 1 glycoprotein genes (PSG) are mainly expressed during human placental development, though their expression has been reported in other normal and pathological tissues, e.g. hydatidiform mole (HM), of distinct origins. However, the molecular components implicated in the regulation of PSG are not well understood. To identify some of the regulatory elements involved in the transcriptional control of PSG expression, the DNA-protein interactions and the basal activities of the TATA-box-less PSG5 promoter were determined in different tissues and cell types. In DNAse-I protection assays, DNA-binding proteins from human term placenta (HTP) protected a region of 27 bp located from nucleotides --150 to --124, overlapping the farthest 5' upstream cap site and resembling an initiator-like element. In electrophoretic mobility shift assays (EMSA), three complexes were detected using nuclear extracts from HTP and an oligonucleotide containing the 27-bp motif. In situ ultraviolet crosslinking analysis of the specific complexes revealed that two proteins of 78.0 kDa and 53.0 kDa are involved in such interactions, in accordance with the bands of 80.0 kDa and 57.5 kDa observed by Southwestern blotting. Competitive EMSA usi...Continue Reading

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Citations

Jun 13, 2003·Gene·Xiuli HuangMark J Cowan
Feb 19, 2013·PloS One·Soledad A CamolottoGraciela M Panzetta-Dutari
Mar 28, 2006·Biochemical and Biophysical Research Communications·Loïc BlanchonVincent Sapin
Apr 4, 1997·The Journal of Biological Chemistry·N P KoritschonerL C Patrito

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