Analyses of IgA1 hinge glycopeptides in IgA nephropathy by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Journal of the American Society of Nephrology : JASN
Y HikiY Kobayashi

Abstract

This study was performed to analyze the structural variety of O-glycans on the IgA1 hinge in IgA nephropathy (IgAN). The IgA1 fragments containing the hinge glycopeptide (33-mer hinge peptide core (HP) + O-glycans) were separated from 13 IgAN patients, eight healthy control subjects, and 11 patients with other primary glomerulonephritides by pyridylethylation, trypsin treatment, and Jacalin affinity chromatography. Because of the use of Jacalin, only the Gal beta 1-3GalNAc residue containing IgA was analyzed. The molecular weights (MW) of the IgA1 fragments treated by the following sequential treatment by exoglycosidases were estimated using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: (1) Sialidase treatment: the MW of the two observed peaks A and B were compatible with (A) HP + 4GalNAc + 4Gal and (B) HP + 5GalNAc + 4Gal. (2) Sialidase and galactosidase: the MW of the two identified peaks a and b were consistent with (a) HP + 4GalNAc and (b) HP + 5GalNAc. (3) Sialidase, galactosidase, and alpha-N-acetylgalactosaminidase. All subjects revealed one peak, indicating the 33-mer IgA1 hinge peptide core. The intensity rate of peak B/A was significantly decreased in the IgAN group (mean +/- SD, 1.01 +...Continue Reading

Citations

Apr 15, 1999·Current Opinion in Pediatrics·T E Hunley, V Kon
Dec 23, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·T KokuboY Kobayashi
Sep 9, 2010·Molecular & Cellular Proteomics : MCP·Kazuo TakahashiMatthew B Renfrow
Jul 30, 1999·Biochemical and Biophysical Research Communications·H IwaseK Hotta
Aug 14, 1999·Journal of the American Society of Nephrology : JASN·A C AllenJ Feehally
Oct 26, 1999·Biochemical and Biophysical Research Communications·H IwaseK Hotta
May 8, 2000·Journal of Mass Spectrometry : JMS·K M Downard
Nov 30, 2000·Journal of the American Society of Nephrology : JASN·Jürgen Floege, John Feehally
Jun 12, 2001·Molecular Immunology·J NovakJ Mestecky
Aug 24, 2001·Journal of the American Society of Nephrology : JASN·Alessandro AmoreRosanna Coppo
Feb 9, 2002·Journal of Clinical Laboratory Analysis·Joseph C K LeungKar Neng Lai
Apr 15, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·H IwaseK Hotta
Jul 20, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ikuko NakamuraYutaka Kobayashi
Apr 15, 1999·Journal of the American Society of Nephrology : JASN·Y HikiY Kobayashi
Mar 4, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Marie-Thérèse LinossierFrançois Berthoux
Sep 5, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Akeyo HorieKenji Maeda
Apr 17, 2004·Kidney International·Rosanna Coppo, Alessandro Amore
May 25, 2004·Seminars in Nephrology·Jonathan BarrattAlice C Smith
Jul 15, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Paul J M van der BoogMohamed R Daha
Feb 9, 2005·Kidney International·Paul J M van der BoogMohamed R Daha
May 25, 2007·Kidney International·A Roos, C van Kooten
Nov 10, 2006·Journal of the American Society of Nephrology : JASN·Alice C SmithJonathan Barratt
Oct 7, 2006·Biochemical and Biophysical Research Communications·Kazuo TakahashiNobuteru Usuda
Nov 10, 2011·Journal of Proteome Research·Kazuo TakahashiMatthew B Renfrow
Nov 9, 2007·Clinical Transplantation·Rosanna CoppoClaudio Ponticelli
Sep 14, 2007·Seminars in Immunopathology·Jonathan BarrattJohn Feehally
Aug 28, 2021·Journal of Clinical Medicine·Yukako OhyamaKazuo Takahashi
Jan 5, 2008·Clinical and Experimental Nephrology·Yoshiyuki HikiSatoshi Sugiyama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.