Analyses of Long Non-Coding RNA and mRNA profiling using RNA sequencing during the pre-implantation phases in pig endometrium

Scientific Reports
Yueying WangMinggang Lei

Abstract

Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated the expression profiles of endometrial tissue on Days 9, 12 and 15 of pregnancy and on Day 12 of non-pregnancy in Yorkshire, and performed a comprehensive analysis of long non-coding RNAs (lncRNAs) in endometrial tissue samples by using RNA sequencing. As a result, 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison. In agreement with previous reports, lncRNAs shared similar characteristics, such as shorter in length, lower in exon number, lower at expression level and less conserved than protein coding transcripts. Bioinformatics analysis showed that DEGs were involved in protein binding, cellular process, immune system process and enriched in focal adhesion, Jak-STAT, FoxO and MAPK signaling pathway. We also found that lncRNAs TCONS_01729386 and TCONS_01325501 may play a vital role in embryo pre-implantation. Furthermore, the expression of FGF7, NMB, COL5A3, S100A8 and PPP1R3D genes were significantly up-regulated at the time of maternal recognition of pregnancy ...Continue Reading

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Citations

Jan 12, 2017·Genetics, Selection, Evolution : GSE·Kévin MuretSandrine Lagarrigue
Jul 1, 2017·Molecular Reproduction and Development·Mallikarjun Bidarimath, Chandrakant Tayade
Feb 16, 2018·Genome Génome / Conseil National De Recherches Canada·Tuanhui RenXiangtao Kang
Aug 15, 2018·Biology of Reproduction·Ashley F GeorgeCarol A Bagnell
Oct 3, 2018·Animal Genetics·G LiangK Li
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Sep 15, 2016·BMC Bioinformatics·Qiu-Zhong ZhouZe Zhang
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Aug 4, 2018·Frontiers in Genetics·Bahareldin A AbdallaXiquan Zhang

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Methods Mentioned

BETA
RNA-seq
PCR
Assay

Software Mentioned

Tophat
PhastCons
PhyloCSF
phyloFit
house
Coding Potential Calculator ( CPC )
Pfam - scan (
SAS
BLAST
phylogenetic frequency

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