Abstract
Organotypic hippocampal slice cultures (OHSCs) are widely used to study the mechanisms of neurodegeneration and neuroprotection. However, there are still controversies about the most appropriate method for quantification of neuronal damage. The response to excitotoxic lesions can be determined by propidium iodide (PI) staining, which labels nuclei of degenerating cells. Semiquantitative measurements of PI staining are based on (1) recording of the propidium iodide (PI) fluorescence intensity or (2) counting of PI positive neuronal nuclei. Here, we investigated OHSCs lesioned by the application of increasing NMDA concentrations (10microM, 50microM and 500microM) at 6 days in vitro (div) for 4h or left untreated, respectively. After 9 div, PI staining was performed and the staining determined in the dentate gyrus and cornu ammonis (CA1) by measurement of PI-fluorescence intensity or by counting PI(+)-nuclei with a confocal laser scanning microscope. The fluorescence intensity of lesioned OHSCs did not show a NMDA concentration dependent difference. In contrast, confocal laser scanning microscopy revealed a significant and dose-dependent increase in the number of PI(+)-nuclei. Linear regression analysis showed a high correlation b...Continue Reading
References
Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Oct 1, 1991·Experimental Neurology·J J VornovJ T Coyle
Jan 1, 1990·Journal of Neuroscience Methods·J D Macklis, R D Madison
Nov 1, 1988·Trends in Neurosciences·B H Gähwiler
Jan 1, 1987·Acta Neuropathologica·K RimvallP G Waser
Aug 1, 1965·Journal of Molecular Biology·M J Waring
Sep 20, 1984·The Journal of Comparative Neurology·J Zimmer, B H Gähwiler
Apr 1, 1984·Neuroscience·B H Gähwiler
Mar 15, 1984·Experientia·B H Gähwiler
Apr 1, 1995·Experimental Neurology·A J BruceM Baudry
Apr 1, 1995·Journal of Neuroscience Methods·U Strasser, G Fischer
Jan 15, 1993·Brain Research. Developmental Brain Research·D MullerL Stoppini
Oct 2, 1995·Brain Research·E BednarskiG Lynch
Aug 14, 1999·Brain Research. Brain Research Protocols·J H LaakeO P Ottersen
Mar 16, 2000·Experimental Neurology·D DingP J Bergold
Sep 13, 2001·The European Journal of Neuroscience·N P HailerF Dehghani
Dec 25, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna RytterTadeusz Wieloch
Apr 17, 2003·Brain Research. Brain Research Protocols·Ilker Y EyüpogluBernd Heimrich
Sep 6, 2003·The European Journal of Neuroscience·Faramarz DehghaniNils P Hailer
Oct 8, 2003·Neuropharmacology·Michael F A FinleyDaniel V Madison
Sep 24, 2004·Experimental Neurology·Faramarz DehghaniNils P Hailer
May 16, 2006·Neuropharmacology·Francesca BosciaMaurizio Taglialatela
Oct 3, 2006·Experimental Neurology·Susanne KreutzFaramarz Dehghani
Jul 23, 2008·Journal of Neuroscience Research·Cornelia VogtFaramarz Dehghani
Oct 7, 2008·Glia·Susanne KreutzFaramarz Dehghani
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi
Citations
May 10, 2012·Journal of Neuroinflammation·Fahim EbrahimiFaramarz Dehghani
Jan 29, 2013·Toxicology·Bin XuZhao-Fa Xu
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kazuhide HayakawaEng H Lo
Sep 21, 2017·International Journal of Molecular Sciences·Tim HohmannFaramarz Dehghani
Jul 29, 2020·Journal of Neurotrauma·Chen Guang YuJames W Geddes
Dec 4, 2019·Frontiers in Neuroscience·Urszula HohmannFaramarz Dehghani
Jan 26, 2021·Molecular Biology Reports·Rafael S FerreiraVictor D A da Silva
Oct 26, 2021·Frontiers in Neuroscience·Urszula HohmannTim Hohmann