Analysing DHPC/DMPC bicelles by diffusion NMR and multivariate decomposition

Biochimica Et Biophysica Acta
Johannes BjörneråsLena Mäler

Abstract

Mixtures of lipids and detergents are known to form bicelles at certain parameter ranges, but many uncertainties remain concerning the details of the phase behaviour of these mixtures and the morphology of the formed lipid assemblies. Here we used nuclear magnetic resonance (NMR) diffusion data in combination with the multivariate processing method speedy component resolution (SCORE) to analyse mixtures of 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) with the relative concentration q=[DMPC]/[DHPC]=0.5 at total lipid concentrations ranging from 15 to 300 mM. With this approach we were able to resolve the heavily overlapping mixture spectra into component spectra and obtained reliable diffusion coefficients for lipid concentrations in the range 15 to 300 mM, although at high concentrations (250-300 mM), non-negativity constraints or overfactoring was required to successfully decompose the data. At 50-300 mM total lipid concentration, the radii estimated from the diffusion coefficient of DMPC indicate assemblies of the appropriate bicelle size, although small size variations exist, while at lower concentrations the morphology appears to change to larger assemblies. Taken ...Continue Reading

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Citations

Dec 15, 2015·Magnetic Resonance in Chemistry : MRC·Jobst LiebauLena Mäler
Jun 19, 2016·Biochimica Et Biophysica Acta·Jobst LiebauLena Mäler
Jun 11, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·K S MineevA S Arseniev
May 23, 2017·Physical Chemistry Chemical Physics : PCCP·Kenji AramakiKyuhei Kitao
Feb 6, 2018·Magnetic Resonance in Chemistry : MRC·Laura CastañarMathias Nilsson
Jul 15, 2020·Materials·Kenji AramakiYoshikazu Konno
Oct 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrew J Y JonesDaniel Nietlispach
Nov 2, 2019·Biochimica Et Biophysica Acta. Biomembranes·E F DudásA Bodor
Dec 16, 2017·Journal of Colloid and Interface Science·Tarek S AwadLaurence S Romsted
Sep 16, 2020·The Journal of Physical Chemistry. B·Hedvika PrimasováJulien Furrer

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