Analysis and antibacterial activity of Nigella sativa essential oil formulated in microemulsion system

Journal of Oleo Science
Hamdy A ShaabanAmal Saad-Hussein

Abstract

The Essential oil (EO) of Nigella sativa (black cumin) was extracted from the crude oil and the volatile constituents were characterized using gas chromatographic analysis. The EO was formulated in water-based microemulsion system and its antibacterial activity against six pathogenic bacteria was evaluated using the agar well diffusion method. This activity was compared with two other well known biologically active natural and synthetic antimicrobials namely eugenol and Ceftriaxone(®). Results showed that N. sativa EO microemulsion was highly effective against S. aureus, B. cereus and S. typhimurium even at the lowest tested concentration of that EO in the microemulsion (100.0 μg/well). Interestingly, the EO microemulsion showed higher antibacterial activity than Ceftriaxone solution against S. typhimurium at 400.0 μg/well and almost comparable activity against E. coli at 500.0 μg/well. No activity was detected for the EO microemulsion against L. monocytogenes and P. aeruginosa. Eugenol which was also formulated in microemulsion was less effective than N. sativa EO microemulsion except against P. aeruginosa. The synthetic antibiotic (Ceftriaxone) was effective against most of the six tested bacterial strains. This work is the f...Continue Reading

References

Nov 1, 1974·The Journal of Infectious Diseases·J C Sadoff, M S Artenstein
Jun 1, 1996·The Journal of Antimicrobial Chemotherapy·M L BarclayK Grimwood
May 29, 2000·Journal of Food Protection·A UlteeE J Smid
Apr 11, 2001·International Journal of Food Microbiology·A Ultee, E J Smid
Jan 7, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K Poole
Jun 21, 2005·BMC Microbiology·Gabriella Kiskó, Sibel Roller
Sep 15, 2005·Foodborne Pathogens and Disease·V SrinivasanS P Oliver
Apr 15, 2006·Clinical Microbiology Reviews·Laura J V Piddock
Nov 28, 2006·Animal Biotechnology·H HarbottleD G White
Jul 3, 2007·Journal of Agricultural and Food Chemistry·Mariateresa CristaniDomenico Trombetta
Jan 29, 2008·Water Research·Gideon P WinwardBruce Jefferson
Jul 11, 2008·Environmental Technology·G P WinwardB Jefferson
Apr 14, 2010·Antimicrobial Agents and Chemotherapy·A MorvanA Le Monnier
Jul 10, 2010·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Eyad M SalemMuhammad A Randhawa
Feb 8, 2011·International Journal of Antimicrobial Agents·Anna SkiadaGeorge L Daikos
Jun 29, 2011·Annals of Clinical Microbiology and Antimicrobials·Bochra KouidhiAmina Bakhrouf
Jul 19, 2011·Journal of Biotechnology·Francesco DonsìGiovanna Ferrari
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Mar 1, 2013·Critical Reviews in Microbiology·Wendy T LangeveldSara A Burt
Mar 30, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Polly Soo Xi YapBeow Chin Yiap
Nov 12, 2013·International Journal of Pharmaceutics·Ioannis LiakosAthanassia Athanassiou

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