Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria

Current Microbiology
Lilian Masoud-LandgrafClemens Kittinger

Abstract

Cystic fibrosis (CF) is the most common hereditary lung disease in the Caucasian population, characterized by viscous bronchial secretion, consecutive defective mucociliary clearance, and unavoidable colonization with microorganisms. Besides Pseudomonas aeruginosa, Staphylococcus aureus is the most common bacterial species colonizing the CF respiratory tract. Under antibiotic pressure S. aureus is able to switch to small colony variants (SCV). These small colony variants can invade epithelial cells, overcome antibiotic therapy inside the cells and can be the starting point for extracellular recolonization. The aim of the present study was the isolation and characterization of S. aureus small colony variants from Austrian cystic fibrosis patients. Samples collected from 147 patients were screened for the presence of S. aureus wild-type and small colony variants. Antibiotic susceptibility testing and determination of the small colony variants causing auxotrophism were performed. Wild-type isolates were assigned to corresponding small colony variants with spa typing. In total, 17 different small colony variant isolates and 12 corresponding wild-type isolates were obtained. 13 isolates were determined thymidine auxotroph, 2 isolate...Continue Reading

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Citations

Apr 20, 2017·Molecular Microbiology·Cyril GuilhenDamien Balestrino
Jun 5, 2019·International Journal of Environmental Research and Public Health·Clemens KittingerGernot Zarfel
Mar 17, 2020·Pediatric Pulmonology·Dilair C de SouzaNelson A Rosario Filho
Jan 14, 2017·Frontiers in Cellular and Infection Microbiology·Judy J J OuSarah Vreugde
Apr 15, 2021·Pathogens and Global Health·Amrita PatilSunil D Saroj
Jul 29, 2021·Microbiology Spectrum·Dilair Camargo de SouzaJussara Kasuko Palmeiro

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Methods Mentioned

BETA
bronchoalveolar lavage

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