Analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial Bacillus amyloliquefaciens SQR9

Microbial Cell Factories
Jiahui ShaoRuifu Zhang

Abstract

The plant growth-promoting rhizobacteria (PGPR) strain Bacillus amyloliquefaciens SQR9, isolated from the cucumber rhizosphere, protects the host plant from pathogen invasion and promotes plant growth through efficient root colonization. The phytohormone indole-3-acetic acid (IAA) has been suggested to contribute to the plant-growth-promoting effect of Bacillus strains. The possible IAA synthetic pathways in B. amyloliquefaciens SQR9 were investigated in this study, using a combination of chemical and genetic analysis. Gene candidates involved in tryptophan-dependent IAA synthesis were identified through tryptophan response transcriptional analysis, and inactivation of genes ysnE, dhaS, yclC, and yhcX in SQR9 led to 86, 77, 55, and 24 % reductions of the IAA production, respectively. The genes patB (encoding a conserved hypothetical protein predicted to be an aminotransferase), yclC (encoding a UbiD family decarboxylase), and dhaS (encoding indole 3-acetaldehyde dehydrogenase), which were proposed to constitute the indole-3-pyruvic acid (IPyA) pathway for IAA biosynthesis, were separately expressed in SQR9 or co-expressed as an entire IAA synthesis pathway cluster in SQR9 and B. subtilis 168, all these recombinants showed incre...Continue Reading

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Citations

Feb 16, 2016·New Biotechnology·Isolde M FrancisDanny Vereecke
Jan 26, 2016·Molecular Plant-microbe Interactions : MPMI·Yunpeng LiuRuifu Zhang
Feb 2, 2018·International Journal of Molecular Sciences·Mengsha LiJun Wu
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Sep 13, 2020·Applied Microbiology and Biotechnology·Chetan KeswaniEstibaliz Sansinenea
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Jul 6, 2019·Journal of Agricultural and Food Chemistry·Daoyi GuoHong Pan
Oct 27, 2021·Applied Microbiology and Biotechnology·Jorge Poveda, Fernando González-Andrés

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Methods Mentioned

BETA
Gene knockout
gene-knockout
genetic modification
reverse transcription PCR
PCR
gene knock-out
ELISA

Software Mentioned

BLASTP

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