Analysis and comparison of electrical pulse parameters for gene electrotransfer of two different cell lines.

The Journal of Membrane Biology
Igor MarjanovicMojca Pavlin

Abstract

Knowledge of the parameters which influence the efficiency of gene electrotransfer has importance for practical implementation of electrotransfection for gene therapy as well as for better understanding of the underlying mechanism. The focus of this study was to analyze the differences in gene electrotransfer and membrane electropermeabilization between plated cells and cells in a suspension in two different cell lines (CHO and B16F1). Furthermore, we determined the viability and critical induced transmembrane voltage (ITV(c)) for both cell lines. In plated cells we obtained relatively little difference in electropermeabilization and gene electrotransfection between CHO and B16F1 cells. However, significant differences between the two cell lines were observed in a suspension. CHO cells exhibited a much higher gene electrotransfection rate compared to B16F1 cells, whereas B16F1 cells reached maximum electropermeabilization at lower electric fields than CHO cells. Both in a suspension and on plated cells, CHO cells had a slightly better survival rate at higher electric fields than B16F1 cells. Calculation of ITV(c) in a suspension showed that, for both electropermeabilization and gene electrotransfection, CHO cells have lower ITV...Continue Reading

References

Feb 19, 2000·Gene Therapy·S Li, L Huang
Apr 28, 2000·Science·M Cavazzana-CalvoA Fischer
Nov 27, 2001·Science·D Ferber
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Muriel GolzioMarie-Pierre Rols
Apr 25, 2003·European Biophysics Journal : EBJ·Blaz ValicDamijan Miklavcic
Jan 1, 1957·Advances in Biological and Medical Physics·H P SCHWAN
Oct 31, 2003·Expert Reviews in Molecular Medicine·Alan L ParkerPaul J Sheridan
Feb 27, 2004·Nature·Marina Cavazzana-CalvoFulvio Mavilio
Mar 29, 2005·Biophysical Journal·Mojca PavlinDamijan Miklavcic
Nov 23, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Erin E Vaughan, David A Dean
Mar 21, 2006·Annals of Biomedical Engineering·G PuciharD Miklavcic
Feb 20, 2007·Current Gene Therapy·Cyril FavardMarie-Pierre Rols
Nov 26, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Adil I DaudRichard Heller
Nov 28, 2008·Electromagnetic Biology and Medicine·Leila TowhidiDamijan Miklavcic
Feb 17, 2009·Nature Medicine·Ronald T MitsuyasuDavid A Cooper
Jul 20, 2010·The Journal of Membrane Biology·M PavlinM Kanduser

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Citations

Jul 25, 2012·The Journal of Membrane Biology·Tomás García-SánchezRamón Bragós
Oct 19, 2012·Radiology and Oncology·Leila TowhidiDamijan Miklavcic
Apr 1, 2014·Biomedical Microdevices·Tomás García-SánchezRamon Bragós
Sep 13, 2011·Bioelectrochemistry·Mojca PavlinMaša Kandušer
Feb 11, 2015·IEEE Transactions on Bio-medical Engineering·Agnese DenziMicaela Liberti
Sep 1, 2015·Bioelectrochemistry·Djamel Eddine ChafaiDamijan Miklavčič
Oct 1, 2013·Bioelectrochemistry·Igor Marjanovič, Tadej Kotnik
Jul 10, 2016·Journal of Surgical Oncology·Neal BhutianiRobert C G Martin

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