Analysis and control of proteolysis of recombinant proteins in Escherichia coli

Advances in Biochemical Engineering/biotechnology
Aleksei Rozkov, Sven-Olof Enfors

Abstract

Proteolysis is one of the reasons for poor production of recombinant proteins in Escherichia coli. Important properties of E. coli proteases, which are relevant for the production of recombinant proteins, are reviewed. Furthermore, various strategies to control the proteolysis of the recombinant proteins are presented. These strategies for control of proteolysis can be applied on various stages of the process: design of more stable protein, a modification of the host cell in respect to proteolytic activity, optimisation of cultivation and downstream processing. However, before implementing these measures the proteolysis rate should be measured in order to calculate a potential benefit of reduced proteolysis rate.

Citations

Jan 1, 2008·Biotechnology & Genetic Engineering Reviews·Yansong MiaoLiwen Jiang
Jul 21, 2010·Journal of Bacteriology·Allison M Michaelis, Zemer Gitai
Jun 18, 2009·Protein Science : a Publication of the Protein Society·Trine ChristensenAshutosh Chilkoti
Jan 6, 2012·Applied Microbiology and Biotechnology·Jerica Sabotič, Janko Kos
Jul 28, 2005·Reproduction, Nutrition, Development·Kahina HaddadiYves Le Roux
Aug 9, 2005·Protein Expression and Purification·Tauseef R ButtMichael R Mattern
Feb 15, 2018·ACS Chemical Biology·Nidhi KakkarWilfred A van der Donk
Aug 28, 2021·International Journal of Molecular Sciences·Bethilda Anne SelvarajWen Siang Tan
Oct 15, 2021·Journal, Genetic Engineering & Biotechnology·Murad MollaevNikita Yabbarov

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