Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp. MZ1T

Environmental Microbiology
Michael S AllenG S Sayler

Abstract

Conditions were developed for the reproducible production, isolation and characterization of a novel microbial extracellular polysaccharide believed to be involved in transient viscous bulking at an industrial wastewater treatment plant. The exopolysaccharide was extracted from cell-free culture supernatants of Thauera sp. strain MZ1T grown on a minimal medium with succinate. The purified polymer was found to be approximately 260 kDa in size by gel-permeation chromatography. The GC-MS analysis of the alditol acetate and per-O-trimethylsilyl methyl glycoside derivatives revealed that the exopolysaccharide was composed of four monosaccharides including: rhamnose, galacturonic acid, N-acetylglucosamine and N-acetylfucosamine. Glucose, which also appeared at low levels, is most likely from a co-eluting glucan. The FTIR and NMR spectroscopic analyses further revealed the presence of esterified component groups on the polymer. These results represent the first published description of a polysaccharide from a member of the genus Thauera, and lay the foundation for a deeper understanding of the factors potentially involved in zoogloeal cluster formation and viscous bulking.

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Citations

Jan 15, 2011·Bioprocess and Biosystems Engineering·Siqing XiaYongming Zhang
Feb 15, 2013·Standards in Genomic Sciences·Ke JiangGary S Sayler
Jul 11, 2012·Water Research·Thomas SeviourYuemei Lin
Jun 16, 2015·Journal of Bioscience and Bioengineering·Di Fang, Cuicui Shi
Jul 28, 2015·Microbiological Research·V N AnupamaB Krishnakumar
Apr 20, 2013·International Journal of Biological Macromolecules·Kuttuvan Valappil SajnaAshok Pandey
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Dorian MieczkowskiPiotr Świątczak
Aug 21, 2013·Bioresource Technology·Chunli WanXiangliang Pan
Oct 17, 2018·Environmental Microbiology·Si-Yu ZhangKonstantinos T Konstantinidis
Feb 1, 2019·Environmental Microbiology·Yossi CohenEdouard Jurkevitch
Nov 28, 2017·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Agnieszka Cydzik-Kwiatkowska, Magdalena Zielińska
Jul 6, 2019·Frontiers in Microbiology·Navreet SuriGerrit Voordouw
Nov 28, 2020·Water Research·Bingdi CaoDongsheng Wang
Apr 28, 2021·Environmental Technology·Vânia S BessaPaula M L Castro
Dec 18, 2014·Environmental Science & Technology·Zhongbo ZhouXiaoshan Jia
Sep 8, 2021·Frontiers in Microbiology·Aparna BanerjeeRajesh K Sani

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