Analysis and inactivation of vha55, the gene encoding the vacuolar ATPase B-subunit in Drosophila melanogaster reveals a larval lethal phenotype.

The Journal of Biological Chemistry
S A DaviesJ A Dow

Abstract

Vacuolar ATPases play major roles in endomembrane and plasma membrane proton transport in eukaryotes. A Drosophila melanogaster cDNA encoding vha55, the 55-kDa vacuolar ATPase (V-ATPase) regulatory B-subunit, was characterized and mapped to 87C2-4 on chromosome 3R. A fly line was identified that carried a single lethal P-element insertion within the coding portion of gene, and its LacZ reporter gene revealed elevated expression in Malpighian tubules, rectum, antennal palps, and oviduct, regions where V-ATPases are believed to play a plasma membrane, rather than an endomembrane, role. The P-element vha55 insertion was shown to be allelic to a known lethal complementation group l(3)SzA (= l(3)87Ca) at 87C, for which many alleles have been described previously. Deletions of the locus have been shown to be larval lethal, whereas point mutations show a range of phenotypes from subvital to embryonic lethal, implying that severe alleles confer a partial dominant negative phenotype. The P-element null allele of vha55 was shown also to suppress ectopic sex combs in Polycomb males, suggesting that transcriptional silencing may be modulated by genes other than those with known homeotic or DNA binding functions.

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R D NelsonS Gluck
Aug 17, 1992·Biochimica Et Biophysica Acta·F J NovakW R Harvey
Feb 1, 1991·Trends in Pharmacological Sciences·N Nelson
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K Kaiser, S F Goodwin
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H Nelson, N Nelson
Oct 1, 1990·Kidney International·D K StoneX S Xie
Dec 25, 1990·Nucleic Acids Research·H H GuG E Dean
Mar 1, 1989·Trends in Biochemical Sciences·N Nelson, L Taiz
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J A Kennison, J W Tamkun
Jan 1, 1988·Nucleic Acids Research·S AotaT Ikemura
Oct 1, 1966·The Journal of Cell Biology·E Anderson, W R Harvey
Jan 26, 1996·The Journal of Biological Chemistry·Q LiuM Forgac

❮ Previous
Next ❯

Citations

Apr 17, 2007·Pflügers Archiv : European journal of physiology·Maurice J Kernan
May 27, 2008·The Journal of Biological Chemistry·Gloria A Martínez-Muñoz, Patricia Kane
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Jan DettmerKarin Schumacher
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Patricia M Kane
Dec 8, 2009·Annual Review of Entomology·Klaus W BeyenbachJulian A T Dow
Jan 1, 1997·Annual Review of Cell and Developmental Biology·T H Stevens, M Forgac
Aug 24, 2012·American Journal of Physiology. Cell Physiology·Aylin R RodanChou-Long Huang
Oct 12, 2010·American Journal of Physiology. Renal Physiology·Julian A T Dow, Michael F Romero
May 19, 2009·The Journal of Experimental Biology·Helmut WieczorekOlga Vitavska
Aug 21, 2013·PloS One·Jianxiu YaoAnna E Whitfield
Jul 22, 2011·Genetics·Amy L AdamsonDennis LaJeunesse
Aug 8, 2014·PLoS Neglected Tropical Diseases·Seokyoung KangGeorge Dimopoulos
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A SözenJ A Dow
May 28, 2010·Kidney International·Gabin SihnMichael Bader
Feb 26, 2009·Genesis : the Journal of Genetics and Development·Diana L van de HoefGabrielle L Boulianne
Dec 21, 2004·Journal of Insect Physiology·E PyzaI A Meinertzhagen
Nov 10, 2015·Nature Neuroscience·Benjamin R RostChristian Rosenmund
Sep 30, 2006·Current Opinion in Plant Biology·Karin Schumacher
Jul 20, 2010·Biochimica Et Biophysica Acta·Sun-Kyung LeeJoohong Ahnn
Jan 23, 2015·Development, Growth & Differentiation·Robert FarkašBernard M Mechler
Sep 4, 2004·Genome Biology·Jing WangJulian A T Dow
Mar 23, 2015·Journal of Insect Physiology·Ismael E Badillo-VargasAnna E Whitfield
Sep 13, 2008·Biochimica Et Biophysica Acta·Sheena Claire Li, Patricia M Kane
Apr 6, 2013·General and Comparative Endocrinology·Shireen-A DaviesJulian A T Dow
Nov 15, 2013·Scientific Reports·Claudia MüllerJuan R Martínez-Morales
Mar 5, 2016·Disease Models & Mechanisms·Berrak UgurHugo J Bellen
Sep 24, 2004·Physiological Reviews·Carsten A WagnerJohn P Geibel
May 31, 2007·Nature Genetics·Venkateswara R ChintapalliJulian A T Dow
Jan 14, 2005·Journal of the American Society of Nephrology : JASN·Alain C JungMarkus Affolter
Jul 4, 2019·Current Opinion in Nephrology and Hypertension·Aylin R Rodan

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.