Analysis and Purification of Synthetic Nucleic Acids Using HPLC

Current Protocols in Nucleic Acid Chemistry
Nanda D Sinha, Kyeong Eun Jung

Abstract

Chromatographic methods have been essential tools for analysis and purification of synthetic oligonucleotides since the 1970s. Significant developments in terms of instruments and stationary phases (media) have been made during the past several decades; among the latest are sub-micron to micron particles for the media, as well as ultra performance liquid chromatography (UPLC). Micron and sub-micron particles have increased product resolution. Applications of recently developed methods such as IP-RP-HPLC and LC-MS have been discussed for analysis, along with use of various methods for purification. Utilization of UPLC has decreased analysis time, increasing the throughput for analysis. Commonly used methods for analysis and purification of synthetic oligonucleotides have been described in this unit.

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Citations

Oct 29, 2018·Current Protocols in Nucleic Acid Chemistry·M Benjamin TurnerByron W Purse
Nov 22, 2018·Current Protocols in Nucleic Acid Chemistry·Minguk SeoMartin Egli
Mar 13, 2019·Current Protocols in Nucleic Acid Chemistry·Ken YamadaFumi Nagatsugi
Jun 4, 2016·Current Protocols in Chemical Biology·Stephanie MackSubha R Das
Jun 2, 2016·Current Protocols in Nucleic Acid Chemistry·Hiroto Fujita, Masayasu Kuwahara
Jun 20, 2017·Current Protocols in Nucleic Acid Chemistry·Andrzej GrajkowskiSerge L Beaucage
Jun 20, 2017·Current Protocols in Nucleic Acid Chemistry·Arindom ChatterjeeAshis K Basu
Jan 28, 2020·Current Protocols in Nucleic Acid Chemistry·Masaaki Akabane-NakataMuthiah Manoharan
Jun 13, 2020·Current Protocols in Nucleic Acid Chemistry·Kacper SkakujChad A Mirkin
Jun 22, 2018·Current Protocols in Nucleic Acid Chemistry·Thomas ZettlXuesong Shi
Feb 2, 2019·Scientific Reports·Anastassia Kanavarioti
Sep 19, 2015·Current Protocols in Nucleic Acid Chemistry·Shigeori Takenaka
Sep 28, 2021·Current Protocols·Muhan HeMaksim Royzen

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