Analysis and quantification of collagen organization with the structure tensor in second harmonic microscopy images of ocular tissues

Applied Optics
Francisco J Ávila, Juan M Bueno

Abstract

The important biological role of collagen-based tissues and the changes produced in the fiber distribution under particular situations (surgery, pathology, external damage, etc.) require tools for the analysis of the collagen organization that might potentially help in early diagnoses. Since collagen structures provide efficient second harmonic generation (SHG) signals, SHG microscopy has emerged as a powerful technique to visualize collagen fibers and qualitatively discriminate normal from abnormal tissues. Here we propose a quantitative method based on the structure tensor to quantify the different organization of collagen patterns in SHG images of ocular tissues. Results show that well-organized collagen distributions present a high degree of isotropy (DoI), a dominant orientation (PO), and a low structural dispersion (SD). On the other hand, the PO vanishes when the collagen tissue is not organized as a consequence of an increase in the SD and a decrease in the DoI. The proposed method is also able to discriminate partially organized samples. The combination of SHG microscopy and the structure tensor is a useful method to objectively classify collagen distributions. Clinical applications of this technique could help in the ...Continue Reading

References

Nov 1, 1978·Biophysical Journal·Y Lanir
Mar 14, 1972·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J DiamantR G Arridge
Jan 1, 1995·Annual Review of Biochemistry·D J Prockop, K I Kivirikko
Jul 31, 1998·Computers in Biology and Medicine·H G Adelmann
Aug 23, 2001·Journal of Biomedical Optics·P J CampagnolaL M Loew
Jun 8, 2004·Journal of Biomechanical Engineering·Gerhard A HolzapfelRay W Ogden
Dec 3, 2004·IEEE Transactions on Pattern Analysis and Machine Intelligence·Josef BigunKenneth Nilsson
Jun 25, 2005·Journal of Cataract and Refractive Surgery·Jorge L AlióAhmed Galal
Mar 1, 2006·Investigative Ophthalmology & Visual Science·Shu-Wen TengChen-Yuan Dong
Jul 20, 2006·Journal of the Royal Society, Interface·T Christian GasserGerhard A Holzapfel
Nov 4, 2006·Journal of Cataract and Refractive Surgery·Naoyuki MorishigeJames V Jester
Nov 24, 2006·Investigative Ophthalmology & Visual Science·Hsin-Yuan TanChen-Yuan Dong
Feb 28, 2007·Investigative Ophthalmology & Visual Science·Naoyuki MorishigeJames V Jester
Jan 1, 1995·IEEE Transactions on Medical Imaging·H Schomberg, J Timmer
Apr 15, 1982·Applied Optics·B F Hochheimer
Jan 5, 2011·Journal of Biomedical Optics·Juan M BuenoPablo Artal
Jun 24, 2011·Biomedical Optics Express·Juan M BuenoPablo Artal
Jan 19, 2012·Biomedical Optics Express·Gaël LatourMarie-Claire Schanne-Klein
May 10, 2012·Investigative Ophthalmology & Visual Science·Wen LoHsin-Yuan Tan
Dec 12, 2012·Journal of Biomedical Optics·Dora Aït-BelkacemSophie Brasselet
Jan 4, 2013·The Laryngoscope·Michael M JohnsRyan C Branski

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Citations

Nov 15, 2017·Scientific Reports·Stefan G StanciuJuan M Bueno
Jun 23, 2016·Journal of Biomedical Optics·Francisco J ÁvilaJuan M Bueno
Jun 20, 2017·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·William J KowalskiBradley B Keller
Jan 24, 2018·Biomedical Optics Express·James A GermannSusana Marcos
Feb 14, 2019·BioMed Research International·Juan M BuenoM Carmen Martínez-García
Jul 12, 2019·Translational Vision Science & Technology·Francisco J ÁvilaJuan M Bueno
Jul 14, 2019·Scientific Reports·Francisco J ÁvilaJuan M Bueno
Jun 19, 2020·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Roberta RenzoJosé L Laus
Nov 6, 2020·Biomedical Optics Express·James P McLeanChristine P Hendon
Dec 8, 2020·Biomedical Optics Express·Luana A ReisAna M de Paula
Mar 9, 2021·Biomedical Optics Express·Juan M BuenoM Carmen Martínez-García

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