Analysis and refinement of 2D single-particle tracking experiments.

Biointerphases
Yannic Kerkhoff, Stephan Block

Abstract

In recent decades, single particle tracking (SPT) has been developed into a sophisticated analytical approach involving complex instruments and data analysis schemes to extract information from time-resolved particle trajectories. Very often, mobility-related properties are extracted from these particle trajectories, as they often contain information about local interactions experienced by the particles while moving through the sample. This tutorial aims to provide a comprehensive overview about the accuracies that can be achieved when extracting mobility-related properties from 2D particle trajectories and how these accuracies depend on experimental parameters. Proper interpretation of SPT data requires an assessment of whether the obtained accuracies are sufficient to resolve the effect under investigation. This is demonstrated by calculating mean square displacement curves that show an apparent super- or subdiffusive behavior due to poor measurement statistics instead of the presence of true anomalous diffusion. Furthermore, the refinement of parameters involved in the design or analysis of SPT experiments is discussed and an approach is proposed in which square displacement distributions are inspected to evaluate the qualit...Continue Reading

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Jan 17, 2021·Biophysical Journal·Benjamin A DaltonItsuo Hanasaki
May 6, 2021·Scientific Reports·Timo KuhnJ Christof M Gebhardt
Jul 23, 2021·Nature Communications·Florian StehrPetra Schwille

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