Analysis and stability study of temozolomide using capillary electrophoresis

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Melinda AndrásiÁlmos Klekner

Abstract

The applicability of micellar electrokinetic capillary chromatography (MEKC) for the analysis of temozolomide (TMZ) and its degradants, 3-methyl-(triazen-1-yl)imidazole-4-carboxamide (MTIC) and 5-amino-imidazole-4-carboxamide (AIC) has been studied. Using short-end injection, the analysis of TMZ and its degradants could be performed within 1.2 min. The obtained precision of migration times was better than 1.6 RSD%, and the limit of quantitation (LOQ) was 0.31-0.93 microg/mL. The therapeutic concentration of TMZ in blood samples can be determined after direct sample injection and conventional on-capillary UV detection. The proposed MEKC method was applied to study the stability of TMZ in water and serum at different pH values. It was established that the half-life of the TMZ in vitro serum at room temperature was 33 min, close to the half-life (28 min) obtained in water at pH 7.9.

Citations

Nov 15, 2012·Analytical and Bioanalytical Chemistry·Ilanna C LopesAna Maria Oliveira-Brett
Jul 6, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Melinda AndrásiAttila Gáspár
Dec 21, 2010·Electrophoresis·Sami El DeebRonald Gust
Dec 13, 2012·CNS Neuroscience & Therapeutics·Zhen-Dong ShiUNKNOWN Chinese Glioma Cooperative Group (CGCG)
Nov 24, 2012·Electrophoresis·Zdeněk Glatz
Jul 12, 2013·Biomedical Chromatography : BMC·Imran AliMohammed Al Za'abi
Mar 29, 2014·Cancer Science·Shigeo AnaiJun-ichi Kuratsu
May 25, 2013·Journal of Pharmaceutical and Biomedical Analysis·Łukasz JedynakJoanna Zagrodzka
Oct 4, 2011·Talanta·Susanne NussbaumerSandrine Fleury-Souverain
Jun 1, 2017·The Analyst·Nicolas GuichardSandrine Fleury-Souverain
Sep 12, 2019·Biomedicines·Hannah StrobelMike-Andrew Westhoff
Dec 14, 2011·Analytical Chemistry·Matthew GeigerMichael T Bowser

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