PMID: 8615456Apr 1, 1996Paper

Analysis and ultrastructural localization of Ehrlichia chaffeensis proteins with monoclonal antibodies

The American Journal of Tropical Medicine and Hygiene
S M ChenD H Walker

Abstract

Ehrlichia chaffeensis, an obligately intracellular bacterium with tropism for monocytes, is the etiologic agent of human monocytic ehrlichiosis. To determine the nature and ultrastructural location of E. chaffeensis antigens, monoclonal antibodies (MAbs) to E. chaffeensis were developed. The MAbs were used for immunofluorescence and Western immunoblotting analysis of the antigens of density gradient-purified ehrlichiae. Monoclonal antibody 6A1 recognized an epitope of a 30-kD protein. This antibody reacted with a strain-specific epitope of E. chaffeensis, Arkansas strain, and did not cross-react with any other ehrlichia tested. Monoclonal antibodies 3C7 and 7C1-B recognized Arkansas strain proteins of 30 and 29 kD and reacted with E. chaffeensis (strain 91HE17) proteins of 31 and 29 kD and an E. canis protein of 30 kD. Lack of reactivity of these two MAbs with E. sennetsu and E. risticii suggests that the epitope is group-specific. Monoclonal antibody 5D11 recognized a 58-kD protein of both strains of E. chaffeensis as well as E. canis, apparently a group-specific, conformation-independent epitope. Monoclonal antibody 7C1-C reacted with 58- and 88-kD proteins of both the Arkansas and 91HE17 strains. Trypsin treatment destroyed ...Continue Reading

Citations

Jun 21, 2002·Veterinary Clinical Pathology·Michelle L. PlierJ. Stephen Dumler
Dec 25, 2004·Infection and Immunity·Vijayakrishna SinguRoman R Ganta
Dec 23, 2003·Infection and Immunity·Roman R GantaStephen K Chapes
Jan 27, 2004·Infection and Immunity·Hui-Min Feng, David H Walker
Jul 2, 1998·Biochemical and Biophysical Research Communications·G R ReddyA R Alleman
Jun 10, 1998·Journal of Clinical Microbiology·M D RavynR C Johnson
Jan 4, 2001·Journal of Clinical Microbiology·J W McBrideD H Walker
Jun 28, 2001·Journal of Clinical Microbiology·A R AllemanM Bélanger

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