Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis

Respirology : Official Journal of the Asian Pacific Society of Respirology
Yuben P MoodleyDino B A Tan

Abstract

Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomar...Continue Reading

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Citations

Mar 19, 2020·Respirology : Official Journal of the Asian Pacific Society of Respirology·Peter M George, Athol U Wells
Sep 6, 2019·Respirology : Official Journal of the Asian Pacific Society of Respirology·Daniel I Sullivan, Daniel J Kass
Oct 20, 2020·Molecular Omics·Tila KhanKoel Chaudhury
Oct 22, 2020·Clinical Science·Kaj E C BloklandJanette K Burgess
Jun 4, 2020·Respiratory Investigation·Daniel S GlassAllison B Reiss

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