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Analysis for indole compounds in urine by high-performance liquid chromatography with fluorometric detection

Clinical Chemistry

Feb 1, 1976

A P Graffeo, Barry L Karger

PMID: 2390

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Abstract

We describe a chromatographic system involving a high-performance chemically-bonded reverse-phase column and fluorescence detection for measurement of indoles in urine. We controlled retention and selectivity by optimizing the methanol content and pH of the mobile phase. Six reference i...read more

Mentioned in this Paper

Chemicals
Fluorescence Spectroscopy
Hydroxytryptamine
Portion of Urine
5-Hydroxytryptophan
Triptans
Indoles
Liquid Chromatography
Hydrogen-Ion Concentration
Hydroxyindoleacetic Acid
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  • Analysis for indole compounds in urine by high-performance liquid chromatography with fluorometric detection

    Clinical Chemistry

    Feb 1, 1976

    A P Graffeo, Barry L Karger

    PMID: 2390

    DOI:

    Abstract

    We describe a chromatographic system involving a high-performance chemically-bonded reverse-phase column and fluorescence detection for measurement of indoles in urine. We controlled retention and selectivity by optimizing the methanol content and pH of the mobile phase. Six reference i...read more

    Mentioned in this Paper

    Chemicals
    Fluorescence Spectroscopy
    Hydroxytryptamine
    Portion of Urine
    5-Hydroxytryptophan

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