Analysis of 10,000 ESTs from lymphocytes of the cynomolgus monkey to improve our understanding of its immune system.

BMC Genomics
Wei-Hua ChenXiao-Ning Wang

Abstract

The cynomolgus monkey (Macaca fascicularis) is one of the most widely used surrogate animal models for an increasing number of human diseases and vaccines, especially immune-system-related ones. Towards a better understanding of the gene expression background upon its immunogenetics, we constructed a cDNA library from Epstein-Barr virus (EBV)-transformed B lymphocytes of a cynomolgus monkey and sequenced 10,000 randomly picked clones. After processing, 8,312 high-quality expressed sequence tags (ESTs) were generated and assembled into 3,728 unigenes. Annotations of these uniquely expressed transcripts demonstrated that out of the 2,524 open reading frame (ORF) positive unigenes (mitochondrial and ribosomal sequences were not included), 98.8% shared significant similarities (E-value less than 1e-10) with the NCBI nucleotide (nt) database, while only 67.7% (E-value less than 1e-5) did so with the NCBI non-redundant protein (nr) database. Further analysis revealed that 90.0% of the unigenes that shared no similarities to the nr database could be assigned to human chromosomes, in which 75 did not match significantly to any cynomolgus monkey and human ESTs. The mapping regions to known human genes on the human genome were described ...Continue Reading

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Apr 4, 2008·Molecular Genetics and Genomics : MGG·Joachim JacobGodelieve Gheysen
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Datasets Mentioned

BETA
DW522370
DW530304

Methods Mentioned

BETA
PCR

Software Mentioned

geneid
phrap
Phred
RAMOS
twinscan
BLAT
CONSED
BLAST
BLASTX
genScan

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