Analysis of a basic drug by on-line solid-phase extraction liquid chromatography/tandem mass spectrometry using a mixed mode sorbent

Rapid Communications in Mass Spectrometry : RCM
C R MalletUwe Neue

Abstract

Two on-line configurations using multiple 6- and 10-port valves were investigated for high-flow on-line extraction of a basic drug in rat plasma and human urine using a reversed-phase and a cation-exchange SPE sorbent. The first configuration studied was a single reversed-phase extraction cartridge (2.1 x 20 mm, 25 microm) using an optimized washing protocol. The results showed that up to 1.5 mL of sample (urine or plasma diluted 1:1) can be injected with limits of quantification (LOQs) as low as 100 pg/mL. The second configuration studied was the combination of a cation exchanger and a reversed-phase cartridge using at-column dilution. The results showed better LOQs than those obtained with the single cartridge at 10 pg/mL with the same injection volume. The mass spectrometer was operated in the multiple reaction monitoring (MRM) mode. All calibration curves gave an average of 5% relative standard deviation (RSD).

References

Jul 17, 1999·Rapid Communications in Mass Spectrometry : RCM· de Biasi VS Jarvis
Feb 12, 2000·Rapid Communications in Mass Spectrometry : RCM·N Van EeckhoutC Van Peteghem
Sep 26, 2000·Biomedical Chromatography : BMC·M Jemal

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Citations

Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S SouverainJ-L Veuthey
Mar 7, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mikaël LeviAlain Pruvost
Aug 23, 2006·Journal of Separation Science·Núria FontanalsUwe D Neue
Jun 13, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Guodong Zhang, Chad E Wujcik
Jan 4, 2003·Rapid Communications in Mass Spectrometry : RCM·Claude R MalletUwe D Neue
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Jan 13, 2006·Therapeutic Drug Monitoring·Paul J Taylor
Dec 8, 2017·Rapid Communications in Mass Spectrometry : RCM·Malorie MellaClaude Mallet

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