Analysis of a new dimeric extradiol dioxygenase from a naphthalenesulfonate-degrading sphingomonad.

Microbiology
Gesche HeissAndreas Stolz

Abstract

A new extradiol dioxygenase was cloned by screening a gene bank from the naphthalenesulfonate-degrading bacterial strain BN6 for colonies with 2,3-dihydroxybiphenyl dioxygenase (DHBPDO) activity. A 1.6 kb DNA fragment was sequenced and an ORF of 954 bp identified. Comparison of the deduced amino acid sequence of DHBPDO II from strain BN6 with previously published sequences showed the closest relationship to a metapyrocatechase (MpcII) from Alcaligenes eutrophus JMP 222. Thus, the enzyme was only distantly related to the main groups of catechol 2,3-dioxygenases or DHBPDOs. The dioxygenase was expressed using a T7 expression vector and the enzymic characteristics of the protein were examined. The enzyme oxidized 2,3-dihydroxybiphenyl, 3-isopropylcatechol, 3-methylcatechol, 4-fluorocatechol and 1,2-dihydroxynaphthalene. Comparison of the UV/visible spectrum of the product formed from 3,5-dichlorocatechol with previous reports suggested that this substrate is oxidized by different extradiol dioxygenases either by proximal or distal ring cleavage. The enzyme required Fe2+ for maximal activity. In contrast to most other extradiol dioxygenases, the enzyme consisted of only two identical subunits.

References

Jan 1, 1992·Annual Review of Microbiology·S HarayamaE L Neidle
Feb 1, 1992·Applied and Environmental Microbiology·R H AdamsD D Focht
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 1, 1986·Applied and Environmental Microbiology·B NörtemannH J Knackmuss
May 1, 1981·Applied and Environmental Microbiology·G M Klecka, D T Gibson
Aug 1, 1994·Applied and Environmental Microbiology·J J Arensdorf, D D Focht
Mar 1, 1984·Applied and Environmental Microbiology·I BartelsW Reineke

❮ Previous
Next ❯

Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·Masayuki SakaiMasao Fukuda
Apr 12, 2003·The Journal of General and Applied Microbiology·Onruthai PinyakongToshio Omori
Dec 31, 2005·Journal of Bioscience and Bioengineering·Kanako TagoMasahito Hayatsu
Aug 19, 2014·Microbial Biotechnology·Daniel Dobslaw, Karl-Heinrich Engesser
Jul 29, 2015·Applied Microbiology and Biotechnology·Martina Kiel, Karl-Heinrich Engesser
Feb 17, 1999·FEMS Microbiology Reviews·A M CookF Junker
Apr 28, 2018·Journal of Bioscience and Bioengineering·Masahiro TakeoSeiji Negoro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.