Analysis of a post-translational steroid induction system for GIGANTEA in Arabidopsis.

BMC Plant Biology
Markus GünlJoanna Putterill

Abstract

To investigate the link between the flowering time gene GIGANTEA (GI) and downstream genes, an inducible GI system was developed in Arabidopsis thaliana L. Heynh. Transgenic Arabidopsis plant lines were generated with a steroid-inducible post-translational control system for GI. The gene expression construct consisted of the coding region of the GI protein fused to that of the ligand binding domain of the rat glucocorticoid receptor (GR). This fusion gene was expressed from the constitutive cauliflower mosaic virus 35S promoter and was introduced into plants carrying the gi-2 mutation. Application of the steroid dexamethasone (DEX) was expected to result in activation of the GI-GR protein and its relocation from the cytoplasm to the nucleus. Application of DEX to the transgenic plant lines rescued the late flowering phenotype conferred by the gi-2 mutation. However, despite their delayed flowering in the absence of steroid, the transgenic lines expressed predicted GI downstream genes such as CONSTANS (CO) to relatively high levels. Nevertheless, increased CO and FLOWERING LOCUS T (FT) transcript accumulation was observed in transgenic plants within 8 h of DEX treatment compared to controls which was consistent with promotion of...Continue Reading

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Citations

Aug 30, 2011·Nature Genetics·Xiaowu WangUNKNOWN Brassica rapa Genome Sequencing Project Consortium
Jan 17, 2012·BMC Plant Biology·Fanny DevoghalaereKarine Myriam David
Feb 13, 2015·Frontiers in Plant Science·Priyanka Mishra, Kishore C Panigrahi
Jan 30, 2021·Molecular Biology Reports·Ali Inayat MallanoYongguang Li

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Methods Mentioned

BETA
immunoprecipitation
transgenic
in
this
PCR

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