PMID: 9188582Jul 1, 1997Paper

Analysis of a recombinant mouse hepatitis virus expressing a foreign gene reveals a novel aspect of coronavirus transcription

Journal of Virology
F FischerP S Masters

Abstract

We have inserted heterologous genetic material into the nonessential gene 4 of the coronavirus mouse hepatitis virus (MHV) in order to test the applicability of targeted RNA recombination for site-directed mutagenesis of the MHV genome upstream of the nucleocapsid (N) gene and to develop further genetic tools for site-directed mutagenesis of structural genes other than N. Initially, a 19-nucleotide tag was inserted into the start of gene 4a of MHV strain A59 with the N gene deletion mutant Alb4 as the recipient virus. In further work, the entire gene for the green fluorescent protein (GFP) was inserted in place of gene 4, creating the currently largest known RNA virus. The expression of GFP was demonstrated by Western blot analysis of infected cell lysates; however, the level of GFP expression was not sufficient to allow detection of fluorescence of viral plaques. Northern blot analysis of transcripts of GFP recombinants showed the expected alteration of the pattern of the nested MHV subgenomic mRNAs. Surprisingly, though, GFP recombinants also produced an RNA species that was the same size as wild-type mRNA4. Analysis of the 5' end of this species revealed that it was actually a collection of mRNAs originating from 10 differen...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 25, 1990·Nucleic Acids Research·O Fichot, M Girard
Jan 1, 1991·Journal of Virology·P B SethnaD A Brian
Jan 1, 1990·Annual Review of Microbiology·M M Lai
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P B SethnaD A Brian
Mar 1, 1985·The Journal of General Virology·M A Skinner, S G Siddell
Jun 1, 1995·Biologicals : Journal of the International Association of Biological Standardization·M GirardA Martin
Apr 1, 1995·Molecular Biotechnology·S Schlesinger
Feb 11, 1994·Science·M ChalfieD C Prasher
Nov 1, 1995·Trends in Biochemical Sciences·A B CubittR Y Tsien
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·T MebatsionK K Conzelmann

❮ Previous
Next ❯

Citations

Mar 29, 2003·Journal of Virology·Lili Kuo, Paul S Masters
May 8, 2009·Journal of Virology·Kelley R HurstPaul S Masters
Jul 24, 2010·Interdisciplinary Perspectives on Infectious Diseases·Jayasri Das Sarma
Jul 19, 2013·Clinical & Developmental Immunology·Dhriti ChatterjeeJayasri Das Sarma
Jun 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hung-Yi Wu, David A Brian
Sep 26, 2014·The Journal of General Virology·Brenna McGruder, Julian L Leibowitz
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·X Shen, P S Masters
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Mar 29, 2003·Journal of Virology·Bert Jan HaijemaPeter J M Rottier
Oct 15, 2003·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Aug 28, 2003·Journal of Virology·Volker ThielStuart G Siddell
Jan 5, 2002·Journal of Virology·Kristopher M CurtisRalph S Baric
Aug 8, 2013·PloS One·Hung-Yi WuNai-Yun Chang
Jul 11, 1998·Journal of Virology·C A de HaanP J Rottier
Jun 11, 1999·Journal of Virology·B Hsue, P S Masters
May 13, 2006·The Journal of General Virology·Alexander O PasternakEric J Snijder
Mar 22, 2007·The Journal of General Virology·Erwin van den BornEric J Snijder
Jul 13, 2000·Virology·W A Miller, G Koev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.