PMID: 7581319Sep 1, 1995Paper

Analysis of a repeat-containing family of Giardia lamblia variant-specific surface protein genes: diversity through gene duplication and divergence

The Journal of Eukaryotic Microbiology
Y Yang, R D Adam

Abstract

Giardia lamblia trophozoites express on their surfaces one of a set of cysteine-rich antigenically variant proteins, called variant-specific surface proteins, which comprise the majority of proteins detected by surface labeling. While these VSP proteins may be immunodominant proteins important in the host immune response to G. lamblia, the ability to switch expression from one VSP to another may provide a means for the trophozoites to avoid the host immune response. The first VSP characterized, VSPA6 (from the A6 clone of the WB isolate, originally termed CRP170), contains 18-23 copies of a 65 amino acid repeat. We have now used the repeat as a probe to isolate from a WBA6 genomic library two genes related to vspA6 (called vspA6-S1, vspA6-S2). Sequence analysis of the vspA6-S1 gene revealed nearly two complete copies of the 195 bp repeat and substantial nucleotide and translated amino acid similarity in the coding regions 5' and 3' to the repeats. The vspA6-S2 gene, while still related, showed greater divergence from vspA6 than vspA6-S1 in the nonrepeat coding region and contained nearly four copies of a 201 bp repeat that was 75% identical to the 195 bp vspA6 repeat. These results suggest that gene duplication followed by dive...Continue Reading

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Citations

Mar 25, 2000·International Journal for Parasitology·R D Adam
Feb 19, 2002·Molecular and Biochemical Parasitology·Yuankai LinTheodore G Clark
Jun 12, 2003·Molecular and Biochemical Parasitology·Zheng WangBrendan Loftus
Jul 4, 2001·Clinical Microbiology Reviews·R D Adam
Jul 12, 2011·Annual Review of Microbiology·César G PruccaHugo D Luján
Jun 2, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Raúl Argüello-GarcíaGuadalupe Ortega-Pierres
Aug 28, 2009·Cellular Microbiology·Cesar G Prucca, Hugo D Lujan

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