Analysis of accumulation patterns and preliminary study on the condensation mechanism of proanthocyanidins in the tea plant [Camellia sinensis

Scientific Reports
Xiaolan JiangTao Xia

Abstract

In the present study, proanthocyanidins were qualitatively and quantitatively identified using hydrolysis and thiolysis assays, NP-HPLC, HPLC-ESI-MS, MALDI-TOF-MS, (1)H-NMR, and (13)C-NMR techniques in different organs of tea plants. The results showed that in leaves, the tri-hydroxyl, cis- and galloylated flavan-3-ols were the main monomeric catechins units, and (epi)catechin was found to be the major unit of polymeric flavan-3-ols when the degree of polymerization was greater than five. In roots, the PAs were found to be abundant, and epicatechin formed the predominant extension unit of oligomeric and polymeric PAs. In order to understand the mechanism of proanthocyanidins polymerization, auto-condensation of the flavan-3-ols was investigated. The results showed that the same trimers (m/z 865) were detected in the extracts of tea plants and in the non-enzymatic in vitro assay, in weak acid as well as weak alkaline solutions at room temperature, when the substrates used were either procyanidin B2 and monomeric flavan-3-ols (epicatechin or catechin), or only procyanidin B2. This suggested that procyanidin B2 not only released carbocation as electrophilic upper units, but also could be used as nucleophilic lower units directly i...Continue Reading

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Citations

Nov 1, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yumei QianTao Xia
Nov 22, 2016·Nature Plants·Chenggang LiuRichard A Dixon
Jan 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Si ChenXiaomin Yu
Jul 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Si ChenXiaomin Yu
Nov 7, 2019·International Journal of Molecular Sciences·Muhammad AnwarLihui Zeng
Jul 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Meei-Ju YangJi-Yuan Liang
Mar 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shiuh-Tsuen HuangJi-Yuan Liang
Dec 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Meei-Ju YangJi-Yuan Liang
Jul 19, 2020·Journal of Agricultural and Food Chemistry·Zhouping FuTao Xia
May 26, 2021·Journal of the Science of Food and Agriculture·Zhidong LvChengwen Shen
Apr 3, 2018·Journal of Agricultural and Food Chemistry·Wenzhao WangTao Xia
Jan 12, 2019·Journal of Agricultural and Food Chemistry·Pauline RousserieLaurence Geny-Denis
Dec 12, 2017·Journal of Agricultural and Food Chemistry·Xinzhen WangYunsheng Wang
Jun 28, 2019·Journal of Agricultural and Food Chemistry·Daniel Esquivel-AlvaradoJess D Reed

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Methods Mentioned

BETA
galloylation
Assay
nuclear magnetic resonance
NMR

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