PMID: 9439382Jan 24, 1998Paper

Analysis of adult otitis media: polymerase chain reaction versus culture for bacteria and viruses

The Annals of Otology, Rhinology, and Laryngology
E M LiedermanG D Ehrlich

Abstract

Recent studies using the polymerase chain reaction (PCR) have identified bacterial and viral genomic sequences in culture-negative pediatric middle ear effusions. To evaluate this technique in adults, 19 effusions were analyzed to compare bacterial and viral culture and PCR detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and adenovirus. Effusions from 4 subjects positive for human immunodeficiency virus (HIV) were analyzed by PCR for HIV virus. Three of 19 effusions were culture-positive for bacteria, and 0 of 19 for viruses. Fifteen of 19 effusions were PCR-positive for bacterial genomic sequences, and 0 of 19 for adenovirus. Thirteen of 15 PCR-positive specimens demonstrated S pneumoniae, 5 of 15 H influenzae, and 0 of 13 M catarrhalis. All 4 effusions from HIV-positive subjects were PCR-positive for HIV. No effusion was culture-positive and PCR-negative. These results confirm that culture-negative middle ear effusions contain genomic sequences from bacterial pathogens. Finding of HIV RNA and DNA in effusion from HIV-positives suggests replicating virus in this fluid.

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Citations

May 19, 2012·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Paulo Henrique WeckwerthMarco Antonio Húngaro Duarte
Mar 16, 2016·The Journal of Laryngology and Otology·R Mills, I Hathorn
Jan 22, 2002·The Laryngoscope·J C Post
Sep 22, 2006·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Shin KariyaKazunori Nishizaki
Jun 7, 2003·Current Allergy and Asthma Reports·William J Doyle, Cuneyt M Alper
May 10, 2003·Clinical and Diagnostic Laboratory Immunology·Shin KariyaKazunori Nishizaki
Apr 13, 2021·Frontiers in Cellular and Infection Microbiology·Shima AboutalebianHossein Mirhendi

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